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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using UBE2E1 Polyclonal antibody (55457-1-AP)

    WB analysis of HEK-293 using 55457-1-AP (same clone as 55457-1-PBS)

    WB result of UBE2E1 antibody (55457-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBE2E1 transfected HEK-293 cells. This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBE2E1 Polyclonal antibody (55457-1-AP)

    IHC staining of human breast cancer using 55457-1-AP (same clone as 55457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55457-1-AP (UBE2E1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using UBE2E1 Polyclonal antibody (55457-1-AP)

    IHC staining of human breast cancer using 55457-1-AP (same clone as 55457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55457-1-AP (UBE2E1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using UBE2E1 Polyclonal antibody (55457-1-AP)

    IF Staining of A431 using 55457-1-AP (same clone as 55457-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using UBE2E1 antibody (55457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 55457-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

UBE2E1 Polyclonal antibody, PBS Only

UBE2E1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55457-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

UBCH6, UBE2E 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55457-1-PBS targets UBE2E1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2E 1 (UBC4/5 homolog, yeast)
Calculated molecular weight 21 kDa
Observed molecular weight21-27 kDa
GenBank accession numberNM_003341
Gene Symbol UBE2E1
Gene ID (NCBI) 7324
RRIDAB_2918357
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51965
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UBE2E1 is a class III E2 ubiquitin conjugating enzyme that belongs to the UBE2E family of E2s, comprising UBE2E1/UBCH6, UBE2E2/UBCH8, and UBE2E3/UBCH9. UBE2E family members share a highly conserved UBC domain but are distinguished from one another by their unique N-terminal extensions. UbE2E1 contains a 50-residue unstructured N-terminal extension rich in serine and lysine residues, which has been shown to interact with USP7 and is essential for USP7-mediated UbE2E1 stabilization. In addition to its ubiquitin conjugating activity, UBE2E1 can also act as an ISG15 conjugating enzyme in vitro, although this activity has not been shown to occur in vivo. UBE2E1 can be covalently modified with either ISG15 or ubiquitin, both of which interfere with the ubiquitin conjugating activity of UBE2E1. UBE2E1 has also been reported to monoubiquitinate histone H2A at K119 in concert with the PRC1 E3 ligase complex. Full-length UBE2E1 is reported to be a dimer, with dimerization mediated by interaction of residues 21-27 from one molecule with the core domain of another molecule, as well as by contacts between a1 of the two interacting molecules. (PMID: 28073915, PMID: 30282802, PMID: 23871895)