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UBE2C Monoclonal antibody

UBE2C Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1C12H8

Cat no : 66087-1-Ig

Synonyms

UBCH10, UBE2C, Ubiquitin carrier protein C, Ubiquitin protein ligase C


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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, U2OS cells, A549 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66087-1-Ig is UBE2C Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBE2C fusion protein Ag19235 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2C
Calculated molecular weight 179 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC016292
Gene symbol UBE2C
Gene ID (NCBI) 11065
RRIDAB_11232220
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBE2C(Ubiquitin-conjugating enzyme E2 C) is also known as UBCH10 and belongs to the ubiquitin-conjugating enzyme family. It is highly expressed in a variety of human primary tumors, and it is able to promote cell proliferation and malignant transformation(PMID:18703417). UBE2C, along with the E3 ligase of the anaphasepromoting complex (APC), catalyzes the ubiquitination of mitotic cyclins A and B, as well as securin. UBE2C is essential for cell cycle progression, and mutation of its active site cysteine confers a dominant-negative phenotype(PMID:17217624).

Protocols

Product Specific Protocols
WB protocol for UBE2C antibody 66087-1-IgDownload protocol
IHC protocol for UBE2C antibody 66087-1-IgDownload protocol
IF protocol for UBE2C antibody 66087-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell Rep Med

Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers

Authors - Bingxin Liu
humanWB

EMBO J

Shared and distinct mechanisms of UBA1 inactivation across different diseases

Authors - Jason C Collins
WB

Elife

Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition.

Authors - Andrew L Hong
humanIHC,IF

Cancer Lett

LINC01194 recruits NUMA1 to promote ubiquitination of RYR2 to enhance malignant progression in triple-negative breast cancer.

Authors - Duanyang Zhai
humanWB

Int J Biol Sci

PRPF6 promotes androgen receptor/androgen receptor-variant 7 actions in castration-resistant prostate cancer cells.

Authors - Wei Liu
humanWB,IHC

FEBS J

Silencing ubiquitin-conjugating enzyme 2C inhibits proliferation and epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma.

Authors - Xianxing Wang
  • KD Validated