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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human lung cancer using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of mouse liver using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of mouse liver using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human lung cancer using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human urothelial carcinoma using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of human urothelial carcinoma using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse stomach tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of mouse stomach using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat liver tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat liver using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat pancreas using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat stomach using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat stomach tissue using UBC Polyclonal antibody (10457-1-AP)

    IHC staining of rat stomach using 10457-1-AP (same clone as 10457-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10457-1-AP (UBC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10457-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

UBC Polyclonal antibody, PBS Only

UBC Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 10457-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10457-1-PBS targets UBC in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0711

Product name: Recombinant human UBC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-265 aa of BC008955

Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE

相同性解析による交差性が予測される生物種
Full Name ubiquitin C
Calculated molecular weight 60 kDa
GenBank accession numberBC008955
Gene Symbol UBC
Gene ID (NCBI) 7316
RRIDAB_2241301
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP0CG48
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ubiquitin (Ub) is a small, highly conserved eukaryotic protein that has an essential role in diverse cellular signaling pathways, including targeting proteins for proteasomal degradation. Cellular Ub is comprised of two distinct pools consisting of free Ub and Ub-substrate conjugates. UbC is a polyubiquitine gene that encodes Ub polyprotein. UbC, as stress-inducible polyubiquitin precursor proteins of around 9-11 monomers, is cleaved into monomeric ubiquitin by cellular deubiquitylating enzymes.

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