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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using UBAP2L Monoclonal antibody (67588-1-Ig)

    WB analysis of K-562 using 67588-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using UBAP2L Monoclonal antibody (67588-1-Ig)

    WB analysis of HEK-293 using 67588-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using UBAP2L Monoclonal antibody (67588-1-Ig)

    WB analysis of LNCaP using 67588-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67588-1-Ig (UBAP2L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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UBAP2L Monoclonal antibody

UBAP2L Monoclonal Antibody for WB, ELISA
Cat No. 67588-1-Ig
Clone No.1E3D1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

Protein NICE-4, Protein NICE 4, NICE4, NICE 4, KIAA0144

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67588-1-Ig targets UBAP2L in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen UBAP2L fusion protein Ag30125 相同性解析による交差性が予測される生物種
Full Name ubiquitin associated protein 2-like
Calculated molecular weight 115 kDa
Observed molecular weight 140-160 kDa
GenBank accession numberBC003170
Gene Symbol UBAP2L
Gene ID (NCBI) 9898
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14157
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ubiquitin-associated protein 2-like (UBAP2L) is a highly conserved protein with an N-terminal ubiquitin-associated (UBA) domain involved in the ubiquitin-proteasome system and aggregate formation induced by proteasome inhibitors. It was originally identified as a human sperm protein that interacts with zona pellucida 3 in human eggs. It can interact with BIM1 to form a complex that modulates hematopoietic stem cell activity. Recent studies have confirmed that UBAP2L function as an oncogene and is associated with various types of cancer, including prostate cancer, glioma, hepatocellular carcinoma (HCC), and colorectal carcinoma. (PMID: 31114027, PMID: 29196913)

Protocols

Product Specific Protocols
WB protocol for UBAP2L antibody 67588-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols