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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UBAC1 Monoclonal antibody (67385-1-Ig)

    WB analysis using 67385-1-Ig (same clone as 67385-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67385-1-Ig (UBAC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67385-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using UBAC1 Monoclonal antibody (67385-1-Ig)

    IF Staining of HeLa using 67385-1-Ig (same clone as 67385-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using UBAC1 antibody (67385-1-Ig, Clone: 2A3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67385-1-PBS in a different storage buffer formulation.

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UBAC1 Monoclonal antibody, PBS Only

UBAC1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67385-1-PBS
Clone No.2A3G7

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

KPC2, UBA domain containing 1, Kip1 ubiquitination-promoting complex protein 2, Glialblastoma cell differentiation-related protein 1, GBDR1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67385-1-PBS targets UBAC1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBAC1 fusion protein Ag13958 相同性解析による交差性が予測される生物種
Full Name UBA domain containing 1
Calculated molecular weight 405 aa, 45 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC004967
Gene Symbol UBAC1
Gene ID (NCBI) 10422
RRIDAB_2882630
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BSL1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

UBAC1(Ubiquitin-associated domain-containing protein 1) is also named as GBDR1, KPC2, UBADC1. It has several putative phosphorylation sites, including 8 CK2 (see 115440) sites, as well as 1 N-glycosylation site and 2 N-myristoylation sites(PMID:10857748). It is required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.Northern blot analysis shows UBAC1 expression with the highest levels in heart and skeletal muscle and lowest level in lung.