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Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of MDA-MB-453s using 25262-1-AP (same clone as 25262-1-PBS)

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of HepG2 using 25262-1-AP (same clone as 25262-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of A375 using 25262-1-AP (same clone as 25262-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    WB analysis of K-562 using 25262-1-AP (same clone as 25262-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 25262-1-AP (UAP1L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UAP1L1 Polyclonal antibody (25262-1-AP)

    IHC staining of human ovary cancer using 25262-1-AP (same clone as 25262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using UAP1L1 Polyclonal antibody (25262-1-AP)

    IHC staining of human ovary cancer using 25262-1-AP (same clone as 25262-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 25262-1-AP (UAP1L1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UAP1L1 Polyclonal antibody (25262-1-AP)

    IF Staining of HepG2 using 25262-1-AP (same clone as 25262-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 25262-1-AP (UAP1L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25262-1-PBS in a different storage buffer formulation.

UAP1L1 Polyclonal antibody, PBS Only

UAP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25262-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.7.-, UDP-N-acetylhexosamine pyrophosphorylase-like protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25262-1-PBS targets UAP1L1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name UDP-N-acteylglucosamine pyrophosphorylase 1-like 1
Calculated molecular weight 57 kDa
Observed molecular weight 57-59 kDa
GenBank accession numberNM_207309
Gene Symbol UAP1L1
Gene ID (NCBI) 91373
RRIDAB_2879993
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3KQV9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

UDP-N-acetylglucosamine pyrophosphorylase-1-like-1 (UAP1L1) is a protein that shares ~59% sequence identity with UDP-N-acetylglucosamine pryophosphorylase-1 (UAP1), which is directly involved in the synthesis of the sugar donor (UDP-GlcNac) for N-acetylglucosamine modification (O-GlcNAclation) of proteins in the cytoplasm, nucleus, and mitochondria, as well as for O- and N-linked protein glycosylation in the endoplasmic reticulum and the Golgi apparatus (PMID: 30097606, 35168617). Human UAP1L1 is also significantly upregulated in a distinct subset of HCC tissues and patients with upregulated expression of UAP1L1 appeared to have poor prognosis (PMID: 33987240).

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