Validation Data Gallery

Filter: AllWBIHC

WB analysis of HepG2 using 15624-1-AP
WB result of U2AF65 antibody (15624-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF65 transfected HepG2 cells.
WB analysis of Jurkat using 15624-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15624-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15624-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 15624-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293T using 15624-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 15624-1-AP (U2AF65 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 15624-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15624-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15624-1-AP (U2AF65 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

U2AF2/U2AF65 Polyclonal antibody

U2AF2/U2AF65 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15624-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, RIP, IHC, ELISA

Synonyms

hU2AF(65), hU2AF65, U2AF2, U2AF65

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HEK-293T cells, HepG2 cells, HeLa cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 9 publications below
IHC	See 1 publications below
IF	See 2 publications below
RIP	See 1 publications below

Product Information

15624-1-AP targets U2AF2/U2AF65 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag8070 Product name: Recombinant human U2AF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 116-471 aa of BC008740 Sequence: IPATALLPTMTPDGLAVTPTPVPVVGSQMTRQARRLYVGNIPFGITEEAMMDFFNAQMRLGGLTQAPGNPVLAVQINQDKNFAFLEFRSVDETTQAMAFDGIIFQGQSLKIRRPHDYQPLPGMSENPSVYVPGVVSTVVPDSAHKLFIGGLPNYLNDDQVKELLTSFGPLKAFNLVKDSATGLSKGYAFCEYVDINVTDQAIAGLNGMQLGDKKLLVQRASVGAKNATLSTINQTPVTLQVPGLMSSQVQMGGHPTEVLCLMNMVLPEELLDDEEYEEIVEDVRDECSKYGLVKSIEIPRPVDGVEVPGCGKIFVEFTSVFDCQKAMQGLTGRKFANRVVVTKYCDPDSYHRRDFW 相同性解析による交差性が予測される生物種
Full Name	U2 small nuclear RNA auxiliary factor 2
Calculated molecular weight	54 kDa
Observed molecular weight	60-65 kDa
GenBank accession number	BC008740
Gene Symbol	U2AF2
Gene ID (NCBI)	11338
RRID	AB_2211330
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P26368
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

U2AF2, also named as U2 auxiliary factor 65 kDa subunit , is a 475 amino acid protein, which belongs to the splicing factor SR family. U2AF2 is Necessary for the splicing of pre-mRNA. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), pre-mRNA, may couple transcription to splicing. The calculated molecular weight of U2AF2 is 53 kDa, but modified U2AF2 is about 65 kDa.

Protocols

Product Specific Protocols
WB protocol for U2AF2/U2AF65 antibody 15624-1-AP	Download protocol
IHC protocol for U2AF2/U2AF65 antibody 15624-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs. Authors - Lei Sun View Article
mouse	IF,RIP	J Clin Invest Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation. Authors - Xue Gong View Article
human	WB	Sci Rep Identification of novel PANDAR protein interaction partners involved in splicing regulation. Authors - N Pospiech View Article
human	WB	iScience Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells. Authors - Mio Iwasaki View Article
human	WB	J Immunol Res Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p. Authors - Li Fang View Article
human	WB	Cell Death Dis RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner. Authors - Shicheng Sun View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 15624-1-AP

WB analysis of Jurkat using 15624-1-AP

WB analysis of HepG2 using 15624-1-AP

WB analysis of HeLa using 15624-1-AP

WB analysis of HEK-293T using 15624-1-AP

WB analysis of HEK-293T using 15624-1-AP

IHC staining of human breast cancer using 15624-1-AP

IHC staining of human breast cancer using 15624-1-AP

U2AF2/U2AF65 Polyclonal antibody

U2AF2/U2AF65 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.

Circular RNA circEsyt2 regulates vascular smooth muscle cell remodeling via splicing regulation.

Identification of novel PANDAR protein interaction partners involved in splicing regulation.

Multi-omics approach reveals posttranscriptionally regulated genes are essential for human pluripotent stem cells.

Circular RNA FOXP1 Induced by ZNF263 Upregulates U2AF2 Expression to Accelerate Renal Cell Carcinoma Tumorigenesis and Warburg Effect through Sponging miR-423-5p.

RNA binding protein NKAP protects glioblastoma cells from ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent manner.