• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    WB analysis of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

    WB result of Tuberin/TSC2 antibody (24601-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Tuberin/TSC2 transfected HeLa cells. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    WB analysis using 24601-1-AP (same clone as 24601-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 24601-1-AP (Tuberin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    IP experiment of SH-SY5Y using 24601-1-AP (same clone as 24601-1-PBS)

    IP result of anti-Tuberin/TSC2 (IP:24601-1-AP, 4ug; Detection:24601-1-AP 1:1000) with SH-SY5Y cells lysate 1080 ug. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    IHC staining of mouse brain using 24601-1-AP (same clone as 24601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24601-1-AP (Tuberin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    IHC staining of human pancreas using 24601-1-AP (same clone as 24601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    IHC staining of human pancreas using 24601-1-AP (same clone as 24601-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 24601-1-AP (Tuberin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    IF Staining of HEK-293 using 24601-1-AP (same clone as 24601-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Tuberin/TSC2 antibody (24601-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Tuberin/TSC2 Polyclonal antibody (24601-1-AP)

    FC experiment of HeLa using 24601-1-AP (same clone as 24601-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Tuberin (24601-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 24601-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Tuberin/TSC2 Polyclonal antibody, PBS Only

Tuberin/TSC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 24601-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24601-1-PBS targets Tuberin/TSC2 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18133

Product name: Recombinant human TSC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1436-1784 aa of BC150300

Sequence: RGYTISDSAPSRRGKRVERDALKSRATASNAEKVPGINPSFVFLQLYHSPFFGDESNKPILLPNESQSFERSVQLLDQIPSYDTHKIAVLYVGEGQSNSELAILSNEHGSYRYTEFLTGLGRLIELKDCQPDKVYLGGLDVCGEDGQFTYCWHDDIMQAVFHIATLMPTKDVDKHRCDKKRHLGNDFVSIVYNDSGEDFKLGTIKGQFNFVHVMVTPLDYECNLVSLQCRKDMEGLVDTSVAKIVSDRNLPFVARQMALHANMASQVHHSRSNPTDIYPSKWIARLRHIKRLRQRICEEAAYSNPSLPLVHPPSHSKAPAQTPAEPTPGYEVGQRKRLISSVEDFTEFV

相同性解析による交差性が予測される生物種
Full Name tuberous sclerosis 2
Calculated molecular weight 1807 aa, 201 kDa
Observed molecular weight 200 kDa
GenBank accession numberBC150300
Gene Symbol TSC2
Gene ID (NCBI) 7249
RRIDAB_2879633
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49815
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TSC2, also named as TSC4, FLJ43106 and LAM, acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. In complex with TSC1, TSC2 inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. TSC2 implicated as a tumor suppressor. It is involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. TSC2 stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors. Mutations in either TSC2 or the related TSC1 (hamartin) gene cause tuberous sclerosis complex (TSC), an autosomal dominant disorder characterized by development of multiple, widespread non-malignant tumors.

||
New chat

Able™

正在加载，请稍候...