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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis using 67952-1-Ig (same clone as 67952-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis of HSC-T6 using 67952-1-Ig (same clone as 67952-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis of 4T1 using 67952-1-Ig (same clone as 67952-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using TrpRS Monoclonal antibody (67952-1-Ig)

    WB analysis of human placenta using 67952-1-Ig (same clone as 67952-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67952-1-Ig (WARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of LNCaP cells using TrpRS Monoclonal antibody (67952-1-Ig)

    IF Staining of LNCaP using 67952-1-Ig (same clone as 67952-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using TrpRS antibody (67952-1-Ig, Clone: 1C6C3 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67952-1-PBS in a different storage buffer formulation.

TrpRS Monoclonal antibody, PBS Only

TrpRS Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67952-1-PBS
Clone No.1C6C3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

GAMMA 2, hWRS, IFI53, IFP53, Interferon induced protein 53, TrpRS, Tryptophan tRNA ligase, tryptophanyl tRNA synthetase, WARS, WRS

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67952-1-PBS targets TrpRS in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag31430

Product name: Recombinant human WARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-368 aa of BC017489

Sequence: MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQI

相同性解析による交差性が予測される生物種
Full Name tryptophanyl-tRNA synthetase
Calculated molecular weight 471 aa, 53 kDa
Observed molecular weight53 kDa
GenBank accession numberBC017489
Gene Symbol WARS1
Gene ID (NCBI) 7453
RRIDAB_2918704
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP23381
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tryptophanyl-tRNA synthetase (WRS) is a cytosolic amino acyl-tRNA synthetase essential for protein synthesis. Full-length WRS is released during bacterial infection and primes the Toll-like receptor 4 (TLR4)-myeloid differentiation factor 2 (MD2) complex to elicit innate immune responses. Inside the nucleus, WRS stimulates DNA-dependent protein kinase, catalytic subunit (DNA-PKcs), activity via its WHEP domain, leading to the phosphorylation and activation of p53 (39) (PMID:30355684).

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