• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TrpRS Polyclonal antibody (16081-1-AP)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TrpRS Polyclonal antibody (16081-1-AP)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TrpRS Polyclonal antibody (16081-1-AP)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TrpRS Polyclonal antibody (16081-1-AP)

    WB analysis using 16081-1-AP (same clone as 16081-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16081-1-AP (TrpRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TrpRS Polyclonal antibody (16081-1-AP)

    IP experiment of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    IP result of anti-TrpRS (IP:16081-1-AP, 4ug; Detection:16081-1-AP 1:500) with HepG2 cells lysate 3000ug. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TrpRS Polyclonal antibody (16081-1-AP)

    IHC staining of human ovary tumor using 16081-1-AP (same clone as 16081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using TrpRS Polyclonal antibody (16081-1-AP)

    IHC staining of human ovary tumor using 16081-1-AP (same clone as 16081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TrpRS Polyclonal antibody (16081-1-AP)

    IHC staining of human colon cancer using 16081-1-AP (same clone as 16081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TrpRS Polyclonal antibody (16081-1-AP)

    IHC staining of human colon cancer using 16081-1-AP (same clone as 16081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TrpRS Polyclonal antibody (16081-1-AP)

    IHC staining of human colon cancer using 16081-1-AP (same clone as 16081-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16081-1-AP (TrpRS antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TrpRS Polyclonal antibody (16081-1-AP)

    IF Staining of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using WARS antibody 16081-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TrpRS Polyclonal antibody (16081-1-AP)

    IF Staining of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using TrpRS antibody (16081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using TrpRS Polyclonal antibody (16081-1-AP)

    FC experiment of HepG2 using 16081-1-AP (same clone as 16081-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug TrpRS Polyclonal antibody (16081-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16081-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TrpRS Polyclonal antibody, PBS Only

TrpRS Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 16081-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

WARS, Interferon-induced protein 53, IFP53, IFI53, hWRS

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16081-1-PBS targets TrpRS in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9079

Product name: Recombinant human WARS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-368 aa of BC017489

Sequence: MPNSEPASLLELFNSIATQGELVRSLKAGNASKDEIDSAVKMLVSLKMSYKAAAGEDYKADCPPGNPAPTSNHGPDATEAEEDFVDPWTVQTSSAKGIDYDKLIVRFGSSKIDKELINRIERATGQRPHHFLRRGIFFSHRDMNQVLDAYENKKPFYLYTGRGPSSEAMHVGHLIPFIFTKWLQDVFNVPLVIQMTDDEKYLWKDLTLDQAYSYAVENAKDIIACGFDINKTFIFSDLDYMGMSSGFYKNVVKIQKHVTFNQVKGIFGFTDSDCIGKISFPAIQAAPSFSNSFPQIFRDRTDIQCLIPCAIDQDPYFRMTRDVAPRIGYPKPALLHSTFFPALQGAQTKMSASDPNSSIFLTDTAKQI

相同性解析による交差性が予測される生物種
Full Name tryptophanyl-tRNA synthetase
Calculated molecular weight 471 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC017489
Gene Symbol WARS1
Gene ID (NCBI) 7453
RRIDAB_2273061
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP23381
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TrpRS is also named as IFI53, WRS, WARS and belongs to the class-I aminoacyl-tRNA synthetase family. It catalyzes activation of tryptophan by ATP and transfer to tRNATrP, ensuring ranslation of the genetic code for tryptophan. TrpRS is thought to be more closely related to TyrRS than to any other class I enzyme, but the significance of this relationship has been questioned (PMID:7743129). It can exsit as a dimer(PMID:7743129). It has 2 isoforms produced by alternative splicing.

||
New chat

Able™

正在加载，请稍候...