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Validation Data Gallery

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  • Western Blot (WB) analysis of Neuro-2a cells using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of Neuro-2a using 20176-1-AP (same clone as 20176-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of mouse skeletal muscle using 20176-1-AP (same clone as 20176-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of mouse brain using 20176-1-AP (same clone as 20176-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using Trappc9,NIBP Polyclonal antibody (20176-1-AP)

    WB analysis of SH-SY5Y using 20176-1-AP (same clone as 20176-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 20176-1-AP (Trappc9,NIBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20176-1-PBS in a different storage buffer formulation.

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Mouse Trappc9,NIBP Polyclonal antibody, PBS Only

Trappc9,NIBP Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20176-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20176-1-PBS targets Trappc9,NIBP in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Trappc9,NIBP fusion protein Ag14018 相同性解析による交差性が予測される生物種
Full Name trafficking protein particle complex 9
Calculated molecular weight 129/250/128 kDa
Observed molecular weight 128 kDa, 139 kDa
GenBank accession numberBC070463
Gene Symbol NIBP/Trappc9
Gene ID (NCBI) 76510
RRIDAB_10694434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ3U0M1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

TRAPPC9, also named as KIAA1882 and NIBP, belongs to the NIBP family. It functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. TRAPPC9 may function in neuronal cells differentiation. It may play a role in vesicular transport from endoplasmic reticulum to Golgi. TRAPPC9 was found in neurons of the cerebral cortex, hippocampus, and deep gray matter. In WB, the MW of TRAPPC9 is 130-140kd. A 250kd maybe the TRAPPC9 undergoes extensive posttranslational modification.