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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis using 66016-1-Ig (same clone as 66016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of Jurkat using 66016-1-Ig (same clone as 66016-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HeLa using 66016-1-Ig (same clone as 66016-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    WB analysis of human liver using 66016-1-Ig (same clone as 66016-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 66016-1-Ig (Transketolase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human colon cancer using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66016-1-Ig (Transketolase antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Transketolase Monoclonal antibody (66016-1-Ig)

    IHC staining of human brain using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 66016-1-Ig(Transketolase antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Transketolase Monoclonal antibody (66016-1-Ig)

    IF Staining of HepG2 using 66016-1-Ig (same clone as 66016-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Transketolase antibody (66016-1-Ig, Clone: 3E2F3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 66016-1-PBS in a different storage buffer formulation.

Transketolase Monoclonal antibody, PBS Only

Transketolase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66016-1-PBS
Clone No.3E2F3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

TKT, TK, EC:2.2.1.1, 3E2F3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66016-1-PBS targets Transketolase in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16779

Product name: Recombinant human TKT protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 274-623 aa of BC009970

Sequence: IYSQIQSKKKILATPPQEDAPSVDIANIRMPSLPSYKVGDKIATRKAYGQALAKLGHASDRIIALDGDTKNSTFSEIFKKEHPDRFIECYIAEQNMVSIAVGCATRNRTVPFCSTFAAFFTRAFDQIRMAAISESNINLCGSHCGVSIGEDGPSQMALEDLAMFRSVPTSTVFYPSDGVATEKAVELAANTKGICFIRTSRPENAIIYNNNEDFQVGQAKVVLKSKDDQVTVIGAGVTLHEALAAAELLKKEKINIRVLDPFTIKPLDRKLILDSARATKGRILTVEDHYYEGGIGEAVSSAVVGEPGITVTHLAVNRVPRSGKPAELLKMFGIDRDAIAQAVRGLITKA

相同性解析による交差性が予測される生物種
Full Name transketolase
Calculated molecular weight 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC009970
Gene Symbol Transketolase
Gene ID (NCBI) 7086
RRIDAB_11064729
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP29401
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Transketolase is a key pentose phosphate shunt enzyme that plays an important role in the production of reducing equivalents and pentose sugars. Decreased activity of transketolase has been found in patients with Alzheimer's disease and thiamine deficiency. In addition, as an important biocatalyst in stereo-specific carbon-carbon bond synthesis, transketolase has been widely used in industry to produce aromatic products.

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