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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Transferrin Monoclonal antibody, PBS Only

Transferrin Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B4C6

Cat no : 66171-1-PBS

Synonyms

Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, Transferrin

Validation Data Gallery

Applications:
  • WB analysis of human plasma using 66171-1-Ig (same clone as 66171-1-PBS)

    WB analysis of human plasma using 66171-1-Ig (same clone as 66171-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

  • WB analysis of fetal human brain using 66171-1-Ig (same clone as 66171-1-PBS)

    WB analysis of fetal human brain using 66171-1-Ig (same clone as 66171-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66171-1-Ig (Transferrin antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy TF Ready-To-Use IHC Kit. TF primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66171-1-PBS targets Transferrin in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Transferrin fusion protein Ag11668 相同性解析による交差性が予測される生物種
Full Name transferrin
Calculated molecular weight 698 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC059367
Gene symbol TF
Gene ID (NCBI) 7018
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Transferrin(TF) is also named as Beta-1 metal-binding globulin, Siderophilin and belongs to the transferrin family. It is a bilobal iron-binding protein that plays the crucial role of binding ferric iron and keeping it in solution, thereby controlling the levels of this important metal. Serum transferrin may have a further role in stimulating cell proliferation.