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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Tnfrsf11b Recombinant monoclonal antibody (83065-2-RR)

    IF Staining of HeLa using 83065-2-RR (same clone as 83065-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Tnfrsf11b antibody (83065-2-RR, Clone: 230325C3 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83065-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using Tnfrsf11b Recombinant monoclonal antibody (83065-2-RR)

    FC experiment of RAW 264.7 using 83065-2-RR (same clone as 83065-2-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse Tnfrsf11b (83065-2-RR, Clone:230325C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83065-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83065-2-RR

    Affinity and Kinetic Characterization of 83065-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83065-2-RR against Mouse Tnfrsf11b were performed. The affinity constant is 18.1 pM.

Mouse Tnfrsf11b Recombinant monoclonal antibody, PBS Only

Tnfrsf11b Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83065-2-PBS
Clone No.230325C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230325C3, OCIF, Opg, Osteoprotegerin, PDB5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83065-2-PBS targets Tnfrsf11b in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0598

Product name: Recombinant mouse Tnfrsf11b protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-401 aa of BC049782.1

Sequence: ETLPPKYLHYDPETGHQLLCDKCAPGTYLKQHCTVRRKTLCVPCPDHSYTDSWHTSDECVYCSPVCKELQSVKQECNRTHNRVCECEEGRYLEIEFCLKHRSCPPGSGVVQAGTPERNTVCKKCPDGFFSGETSSKAPCRKHTNCSTFGLLLIQKGNATHDNVCSGNREATQKCGIDVTLCEEAFFRFAVPTKIIPNWLSVLVDSLPGTKVNAESVERIKRRHSSQEQTFQLLKLWKHQNRDQEMVKKIIQDIDLCESSVQRHLGHANLTTEQLRALMESLPGKKISPEEIERTRKTCKSSEQLLKLLSLWRIKNGDQDTLKGLMYALKHLKTSHFPKTVTHSLRKTMRFLHSFTMYRLYQKLFLEMIGNQVQSVKISCL

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
Calculated molecular weight46KD
GenBank accession numberBC049782.1
Gene Symbol Tnfrsf11b
Gene ID (NCBI) 18383
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ6PI12
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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