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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Tnfrsf11b Recombinant monoclonal antibody (83065-2-RR)

    IF Staining of HeLa using 83065-2-RR (same clone as 83065-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Tnfrsf11b antibody (83065-2-RR, Clone: 230325C3 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83065-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of RAW 264.7 cells using Tnfrsf11b Recombinant monoclonal antibody (83065-2-RR)

    FC experiment of RAW 264.7 using 83065-2-RR (same clone as 83065-2-PBS)

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse Tnfrsf11b (83065-2-RR, Clone:230325C3) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83065-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83065-2-RR

    Affinity and Kinetic Characterization of 83065-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83065-2-RR against Mouse Tnfrsf11b were performed. The affinity constant is 18.1 pM.

Mouse Tnfrsf11b Recombinant monoclonal antibody, PBS Only

Tnfrsf11b Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83065-2-PBS
Clone No.230325C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

230325C3, OCIF, Opg, Osteoprotegerin, PDB5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83065-2-PBS targets Tnfrsf11b in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0598

Product name: Recombinant mouse Tnfrsf11b protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-401 aa of BC049782.1

Sequence: ETLPPKYLHYDPETGHQLLCDKCAPGTYLKQHCTVRRKTLCVPCPDHSYTDSWHTSDECVYCSPVCKELQSVKQECNRTHNRVCECEEGRYLEIEFCLKHRSCPPGSGVVQAGTPERNTVCKKCPDGFFSGETSSKAPCRKHTNCSTFGLLLIQKGNATHDNVCSGNREATQKCGIDVTLCEEAFFRFAVPTKIIPNWLSVLVDSLPGTKVNAESVERIKRRHSSQEQTFQLLKLWKHQNRDQEMVKKIIQDIDLCESSVQRHLGHANLTTEQLRALMESLPGKKISPEEIERTRKTCKSSEQLLKLLSLWRIKNGDQDTLKGLMYALKHLKTSHFPKTVTHSLRKTMRFLHSFTMYRLYQKLFLEMIGNQVQSVKISCL

相同性解析による交差性が予測される生物種
Full Name tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)
Calculated molecular weight46KD
GenBank accession numberBC049782.1
Gene Symbol Tnfrsf11b
Gene ID (NCBI) 18383
RRIDAB_3670789
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ6PI12
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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