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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse heart tissue using Titin Polyclonal antibody (27867-1-AP)

    IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Titin Polyclonal antibody (27867-1-AP)

    IHC staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Titin Polyclonal antibody (27867-1-AP)

    IHC staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using Titin Polyclonal antibody (27867-1-AP)

    IHC staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 27867-1-AP (Titin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Titin Polyclonal antibody (27867-1-AP)

    IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse skeletal muscle tissue using Titin Polyclonal antibody (27867-1-AP)

    IF Staining of mouse skeletal muscle using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Titin Polyclonal antibody (27867-1-AP)

    IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using Titin Polyclonal antibody (27867-1-AP)

    IF Staining of mouse heart using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Titin antibody (27867-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Titin Polyclonal antibody (27867-1-AP)

    IF Staining of NIH/3T3 using 27867-1-AP (same clone as 27867-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Titin antibody (27867-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27867-1-PBS in a different storage buffer formulation.

Titin Polyclonal antibody, PBS Only

Titin Polyclonal Antibody for IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 27867-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

TTN

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27867-1-PBS targets Titin in IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27496

Product name: Recombinant human TTN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 33738-33969 aa of NM_001256850

Sequence: MFKSIHEKVSKISETKKSDQKTTESTVTRKTEPKAPEPISSKPVIVTGLQDTTVSSDSVAKFAVKATGEPRPTAIWTKDGKAITQGGKYKLSEDKGGFFLEIHKTDTSDSGLYTCTVKNSAGSVSSSCKLTIKAIKDTEAQKVSTQKTSEITPQKKAVVQEEISQKALRSEEIKMSEAKSQEKLALKEEASKVLISEEVKKSAATSLEKSIVHEEITKTSQASEEVRTHAEIK

相同性解析による交差性が予測される生物種
Full Name titin
Calculated molecular weight 3816 kDa
GenBank accession numberNM_001256850
Gene Symbol Titin
Gene ID (NCBI) 7273
RRIDAB_2880998
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WZ42
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Titin, or connectin, is a giant muscle protein expressed in the cardiac and skeletal muscles that spans half of the sarcomere from Z line to M line. It plays a key role in muscle contraction and relaxation, maintaining the structural integrity of the myotome(PMID: 11846417). Mutations in Titin are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma(PMID: 15802564).

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