A172 cells were subjected to SDS PAGE followed by western blot with 85882-2-RR (Timp2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85882-2-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 85882-2-RR (Timp2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85882-2-PBS in a different storage buffer formulation.

Sandwich ELISA standard curve of MP02176-3, Mouse Timp2 Recombinant Matched Antibody Pair - PBS only. 85882-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2960. 85882-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

Mouse lung tissue (3 lungs in 1-2 mm pieces in 100 mL of medium) was cultured for 5 days in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse Timp2, and measured 53.04 ng/mL.

Serum of sixteen mice was measured. The mouse Timp2 concentration of detected samples was determined to be 1,122.15 ng/mL with a range of 933.30 - 1,234.19 ng/mL.