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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    WB analysis using 18304-1-AP (same clone as 18304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    WB analysis of HeLa using 18304-1-AP (same clone as 18304-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    WB analysis using 18304-1-AP (same clone as 18304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    WB analysis using 18304-1-AP (same clone as 18304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    WB analysis of A431 using 18304-1-AP (same clone as 18304-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    WB analysis of human peripheral blood platelets using 18304-1-AP (same clone as 18304-1-PBS)

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    IHC staining of human lung cancer using 18304-1-AP (same clone as 18304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    IHC staining of human lung cancer using 18304-1-AP (same clone as 18304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    IHC staining of human placenta using 18304-1-AP (same clone as 18304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    IHC staining of human placenta using 18304-1-AP (same clone as 18304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    IHC staining of human liver using 18304-1-AP (same clone as 18304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Thrombospondin 1 Polyclonal antibody (18304-1-AP)

    IHC staining of human liver using 18304-1-AP (same clone as 18304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18304-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Thrombospondin 1 Polyclonal antibody, PBS Only

Thrombospondin 1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18304-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

THBS1, Thrombospondin-1, Glycoprotein G, THBS, THBS 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18304-1-PBS targets Thrombospondin 1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name thrombospondin 1
Calculated molecular weight 129 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC028145
Gene Symbol Thrombospondin 1
Gene ID (NCBI) 7057
RRIDAB_2201959
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07996
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

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