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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Thrombopoietin Recombinant antibody (83549-7-RR)

    WB analysis using 83549-7-RR (same clone as 83549-7-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Thrombopoietin Recombinant antibody (83549-7-RR)

    WB analysis using 83549-7-RR (same clone as 83549-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83549-7-RR (Thrombopoietin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Thrombopoietin Recombinant antibody (83549-7-RR)

    IHC staining of mouse liver using 83549-7-RR (same clone as 83549-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Thrombopoietin Recombinant antibody (83549-7-RR)

    IHC staining of mouse liver using 83549-7-RR (same clone as 83549-7-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83549-7-RR (Thrombopoietin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83549-7-PBS in a different storage buffer formulation.

Mouse Thrombopoietin Recombinant monoclonal antibody, PBS Only

Thrombopoietin Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 83549-7-PBS
Clone No.250023B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Thpo, Mpllg, TPO

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83549-7-PBS targets Thrombopoietin in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0678

Product name: Recombinant mouse Thrombopoietin protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 22-356 aa of NM_001173505.1

Sequence: SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET

相同性解析による交差性が予測される生物種
Full Name thrombopoietin
Calculated molecular weight38KD
Observed molecular weight38 kDa
GenBank accession numberNM_001173505.1
Gene Symbol Thrombopoietin
Gene ID (NCBI) 21832
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP40226
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs).

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