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Validation Data Gallery

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  • Western Blot (WB) analysis of H69/H146 cells using Teneurin 1 Polyclonal antibody (21696-1-AP)

    WB analysis of H69/H146 cells using 21696-1-AP (same clone as 21696-1-PBS)

    WB result of anti-ODZ1 (21696-1-AP) with two cell lines by Dr. Petrini, Iacopo. This data was developed using the same antibody clone with 21696-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Teneurin 1 Polyclonal antibody (21696-1-AP)

    WB analysis using 21696-1-AP (same clone as 21696-1-PBS)

    Various lysates were subjected to SDS page followed by western blot with 21696-1-AP (ODZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Teneurin 1 Polyclonal antibody (21696-1-AP)

    IHC staining of human brain using 21696-1-AP (same clone as 21696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21696-1-AP (ODZ1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using Teneurin 1 Polyclonal antibody (21696-1-AP)

    IHC staining of human brain using 21696-1-AP (same clone as 21696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21696-1-AP (ODZ1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Teneurin 1 Polyclonal antibody (21696-1-AP)

    IHC staining of mouse brain using 21696-1-AP (same clone as 21696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21696-1-AP (Teneurin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21696-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Teneurin 1 Polyclonal antibody (21696-1-AP)

    IHC staining of mouse brain using 21696-1-AP (same clone as 21696-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21696-1-AP (Teneurin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21696-1-PBS in a different storage buffer formulation.

Teneurin 1 Polyclonal antibody, PBS Only

Teneurin 1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21696-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ODZ1, Protein Odd Oz/ten-m homolog 1, Ten 1, TEN M1, Ten-1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21696-1-PBS targets Teneurin 1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16289

Product name: Recombinant human ODZ1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-280 aa of BC140783

Sequence: MEQTDCKPYQPLPKVKHEMDLAYTSSSDESEDGRKPRQSYNSRETLHEYNQELRMNYNSQSRKRKEVEKSTQEMEFCETSHTLCSGYQTDMHSVSRHGYQLEMGSDVDTETEGAASPDHALRMWIRGMKSEHSSCLSSRANSALSLTDTDHERKSDGENGFKFSPVCCDMEAQAGSTQDVQSSPHNQFTFRPLPPPPPPPHACTCARKPPPAADSLQRRSMTTRSQPSPAAPAPPTSTQDSVHLHNSWVLNSNIPLETRHFLFKHGSGSSAIFSAASQNY

相同性解析による交差性が予測される生物種
Full Name odz, odd Oz/ten-m homolog 1(Drosophila)
Calculated molecular weight 2732 aa, 306 kDa
Observed molecular weight305 kDa
GenBank accession numberBC140783
Gene Symbol Teneurin 1
Gene ID (NCBI) 10178
RRIDAB_10858625
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKZ4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ODZ1 (also known as teneurin-1, TENM1), which plays a key role in central nervous system ontogenesis. ODZ1 is upregulated in Glioblastoma cells through a Stat3-mediated pathway activated by IL-6, which is released by tumor-associated monocytes. In addition, a hypoxic microenvironment regulates GBM tumor cell migration in part by inducing ODZ1 through hypomethylation of a CpG island in the ODZ1 promoter.

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