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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using Tenascin-X Polyclonal antibody (13595-1-AP)

    WB analysis of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Tenascin-X Polyclonal antibody (13595-1-AP)

    WB analysis using 13595-1-AP (same clone as 13595-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using Tenascin-X Polyclonal antibody (13595-1-AP)

    WB analysis of HT-1080 using 13595-1-AP (same clone as 13595-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 13595-1-AP (Tenascin-X antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using Tenascin-X Polyclonal antibody (13595-1-AP)

    IP experiment of mouse liver using 13595-1-AP (same clone as 13595-1-PBS)

    IP result of anti-Tenascin-X (IP:13595-1-AP, 4ug; Detection:13595-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using Tenascin-X Polyclonal antibody (13595-1-AP)

    IHC staining of human kidney using 13595-1-AP (same clone as 13595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung tissue using Tenascin-X Polyclonal antibody (13595-1-AP)

    IHC staining of human lung using 13595-1-AP (same clone as 13595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Tenascin-X Polyclonal antibody (13595-1-AP)

    IHC staining of human breast cancer using 13595-1-AP (same clone as 13595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using Tenascin-X Polyclonal antibody (13595-1-AP)

    IHC staining of human liver using 13595-1-AP (same clone as 13595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using Tenascin-X Polyclonal antibody (13595-1-AP)

    IHC staining of human heart using 13595-1-AP (same clone as 13595-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 13595-1-AP (Tenascin-X antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13595-1-PBS in a different storage buffer formulation.

Tenascin-X Polyclonal antibody, PBS Only

Tenascin-X Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13595-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13595-1-PBS targets Tenascin-X in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4501

Product name: Recombinant human TNXB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 321-673 aa of BC033740

Sequence: PGSAVDYPLHDLVLHTNYTATVRGLRGPNLTSPASITFTTGLEAPRDLEAKEVTPRTALLTWTEPPVRPAGYLLSFHTPGGQNQEILLPGGITSHQLLGLFPSTSYNARLQAMWGQSLLPPVSTSFTTGGLRIPFPRDCGEEMQNGAGASRTSTIFLNGNRERPLNVFCDMETDGGGWLVFQRRMDGQTDFWRDWEDYAHGFGNISGEFWLGNEALHSLTQAGDYSMRVDLRAGDEAVFAQYDSFHVDSAAEYYRLHLEGYHGTAGDSMSYHSGSVFSARDRDPNSLLISCAVSYRGAWWYRNCHYANLNGLYGSTVDHQGVSWYHWKGFEFSVPFTEMKLRPRNFRSPAGGG

相同性解析による交差性が予測される生物種
Full Name tenascin XB
Calculated molecular weight 4289 aa, 464 kDa
Observed molecular weight 75 kDa, 140 kDa
GenBank accession numberBC033740
Gene Symbol TNXB
Gene ID (NCBI) 7148
RRIDAB_10644124
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP22105
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Tenascin-X (TNXB) is an extracellular matrix glycoprotein predominantly located in the outer reticular lamina of the basement membrane (BM) (PMID: 23768946). It interacts with many other ECM proteins and it accelerates collagen fibrillogenesis in vitro. TNXB plays a role in interactions between cell and ECM, antiadhesive effect, inhibiting cell migration, and in maintaining homeostasis of the extracellular matrix. Deficiency of TNXB has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This antibody is raised against human TNXB and detects a band of about 75 kDa, which probably represents a proteolytic cleaved form of human TNXB (PMID: 17263730). But the serum form of tenascin-X is a 140-kD protein probably resulting from proteolytic cleavage or alternative splicing of tenascin-X(PMID: 16567571, 11642233).

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