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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Talin-1 Monoclonal antibody (67992-1-Ig)

    WB analysis using 67992-1-Ig (same clone as 67992-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using Talin-1 Monoclonal antibody (67992-1-Ig)

    WB analysis of HeLa using 67992-1-Ig (same clone as 67992-1-PBS)

    WB result of Talin-1 antibody (67992-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Talin-1 transfected HeLa cells. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood platelets using Talin-1 Monoclonal antibody (67992-1-Ig)

    WB analysis of human peripheral blood platelets using 67992-1-Ig (same clone as 67992-1-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Talin-1 Monoclonal antibody (67992-1-Ig)

    IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Talin-1 Monoclonal antibody (67992-1-Ig)

    IHC staining of human colon cancer using 67992-1-Ig (same clone as 67992-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using Talin-1 Monoclonal antibody (67992-1-Ig)

    IF Staining of A549 using 67992-1-Ig (same clone as 67992-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using Talin-1 antibody (67992-1-Ig, Clone: 2E12D10 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using Talin-1 Monoclonal antibody (67992-1-Ig)

    FC experiment of HeLa using 67992-1-Ig (same clone as 67992-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Talin-1 Monoclonal antibody (67992-1-Ig, Clone:2E12D10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67992-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Talin-1 Monoclonal antibody, PBS Only

Talin-1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67992-1-PBS
Clone No.2E12D10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

2E12D10, ILWEQ, KIAA1027, talin 1, talin1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67992-1-PBS targets Talin-1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag5937

Product name: Recombinant human TLN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 2193-2541 aa of BC042923

Sequence: GNSCRQEDVIATANLSRRAIADMLRACKEAAYHPEVAPDVRLRALHYGRECANGYLELLDHVLLTLQKPSPELKQQLTGHSKRVAGSVTELIQAAEAMKGTEWVDPEDPTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMVAAATNNLCEAANAAVQGHASQEKLISSAKQVAASTAQLLVACKVKADQDSEAMKRLQAAGNAVKRASDNLVKAAQKAAAFEEQENETVVVKEKMVGGIAQIIAAQEEMLRKERELEEARKKLAQIRQQQYKFLPSELRDEH

相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight 270 kDa
GenBank accession numberBC042923
Gene Symbol Talin-1
Gene ID (NCBI) 7094
RRIDAB_2918741
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9Y490
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

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