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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TYW3 Polyclonal antibody

TYW3 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29213-1-AP

Synonyms

C1orf171, FLJ40918, tRNA yW synthesizing protein 3, TYW3

Validation Data Gallery

Applications:
  • WB analysis using 29213-1-AP

    WB analysis using 29213-1-AP

    U-251 cell lysates were subjected to SDS PAGE followed by western blot with 29213-1-AP (TYW3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 29213-1-AP

    IF Staining of U2OS using 29213-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using TYW3 antibody (29213-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inU-251 cells
Positive IF detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy TYW3 Ready-To-Use IHC Kit. TYW3 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

29213-1-AP targets TYW3 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TYW3 fusion protein Ag30156 相同性解析による交差性が予測される生物種
Full Name tRNA-yW synthesizing protein 3 homolog (S. cerevisiae)
Calculated molecular weight 30 kDa
GenBank accession numberNM_138467
Gene symbol TYW3
Gene ID (NCBI) 127253
RRIDAB_2918250
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TYW3 antibody 29213-1-APDownload protocol
IF protocol for TYW3 antibody 29213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols