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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TYW1B Polyclonal antibody

TYW1B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17665-1-AP

Synonyms

NCRNA00069, RSAFD2, TYW1B

Validation Data Gallery

Applications:
  • WB analysis of mouse liver using 17665-1-AP

    WB analysis of mouse liver using 17665-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17665-1-AP (TYW1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 17665-1-AP

    WB analysis of MCF-7 using 17665-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 17665-1-AP (TYW1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 17665-1-AP

    IHC staining of human ovary using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 17665-1-AP

    IHC staining of human ovary using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung using 17665-1-AP

    IHC staining of human lung using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 17665-1-AP

    IHC staining of human lung using 17665-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 17665-1-AP (TYW1B antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman ovary tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17665-1-AP targets TYW1B in WB, IHC, ELISA applications and shows reactivity with mouse, human samples.

Tested Reactivity mouse, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TYW1B fusion protein Ag11947 相同性解析による交差性が予測される生物種
Full Name tRNA-yW synthesizing protein 1 homolog B (non-protein coding)
Calculated molecular weight 668 aa, 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberBC068520
Gene symbol TYW1B
Gene ID (NCBI) 441250
RRIDAB_2211307
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TYW1B antibody 17665-1-APDownload protocol
IHC protocol for TYW1B antibody 17665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols