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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TYRO3 Polyclonal antibody (28513-1-AP)

    WB analysis using 28513-1-AP (same clone as 28513-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28513-1-AP (TYRO3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of human colon cancer using 28513-1-AP (same clone as 28513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of mouse brain using 28513-1-AP (same clone as 28513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TYRO3 Polyclonal antibody (28513-1-AP)

    IHC staining of mouse brain using 28513-1-AP (same clone as 28513-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28513-1-AP (TYRO3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using TYRO3 Polyclonal antibody (28513-1-AP)

    IF Staining of HCT 116 using 28513-1-AP (same clone as 28513-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using TYRO3 antibody (28513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28513-1-PBS in a different storage buffer formulation.

TYRO3 Polyclonal antibody, PBS Only

TYRO3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28513-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Brt, BYK, Dtk, EC:2.7.10.1, RSE

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28513-1-PBS targets TYRO3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29540

Product name: Recombinant human TYRO3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 451-596 aa of BC049368

Sequence: LRKRRKETRFGQAFDSVMARGEPAVHFRAARSFNRERPERIEATLDSLGISDELKEKLEDVLIPEQQFTLGRMLGKGEFGSVREAQLKQEDSSFVKVAVKMLKADIIASSDIEEFLREAACMKEFDHPHVAKLVGVSLRSRAKGRL

相同性解析による交差性が予測される生物種
Full Name TYRO3 protein tyrosine kinase
Calculated molecular weight 97 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC049368
Gene Symbol TYRO3
Gene ID (NCBI) 7301
RRIDAB_2881162
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06418
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TYRO3 has a structure includes a cytoplasmic domain and an extracellular ligand binding region. And ligand binding at the cell surface induces dimerization and autophosphorylation of TYRO3 on its intracellular domain that provides docking sites for downstream signaling molecules. TYRO3 is associated with many physiological processes including cell survival, migration and differentiation. It has been reported that TYRO3 can be expressed in various regions of the mammalian central nervous system. 28513-1-AP antibody recognizes the 97 kDa and 140 kDa protein which might be the glycosylated form similar to the papers.((PMID: 8873131, 23354874, 21539887)

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