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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TYMS Recombinant monoclonal antibody (86643-1-RR)

    WB analysis using 86643-1-RR (same clone as 86643-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86643-1-RR (TYMS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86643-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using TYMS Recombinant monoclonal antibody (86643-1-RR)

    IHC staining of human rectal cancer using 86643-1-RR (same clone as 86643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 86643-1-RR (TYMS antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86643-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using TYMS Recombinant monoclonal antibody (86643-1-RR)

    IHC staining of human pancreas cancer using 86643-1-RR (same clone as 86643-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 86643-1-RR (TYMS antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86643-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TYMS Recombinant monoclonal antibody (86643-1-RR)

    IF Staining of HepG2 using 86643-1-RR (same clone as 86643-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TYMS antibody (86643-1-RR, Clone: 251532A1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86643-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86643-1-RR

    Affinity and Kinetic Characterization of 86643-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86643-1-RR against Human TYMS were performed. The affinity constant is 0.284 nM.

TYMS Recombinant monoclonal antibody, PBS Only

TYMS Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86643-1-PBS
Clone No.251532A1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Thymidylate synthase, DKCD, EC:2.1.1.45, HsT422, OK/SW-cl.29

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86643-1-PBS targets TYMS in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag7027

Product name: Recombinant human TYMS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC002567

Sequence: MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV

相同性解析による交差性が予測される生物種
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight36 kDa
GenBank accession numberBC002567
Gene Symbol TYMS
Gene ID (NCBI) 7298
RRIDAB_3745024
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP04818
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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