×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    WB analysis using 15047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    WB analysis of HEK-293 using 15047-1-AP

    WB result of Thymidylate synthase antibody (15047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Thymidylate synthase transfected HEK-293 cells.

  • Western Blot (WB) analysis of HEK-293T cells using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    WB analysis of HEK-293T using 15047-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    IP experiment of HeLa using 15047-1-AP

    IP result of anti-Thymidylate synthase (IP:15047-1-AP, 3ug; Detection:15047-1-AP 1:600) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    IHC staining of human endometrial cancer using 15047-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    IHC staining of human endometrial cancer using 15047-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    IHC staining of human colon cancer using 15047-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15047-1-AP (Thymidylate synthase antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Thymidylate synthase Polyclonal antibody (15047-1-AP)

    IF Staining of HeLa using 15047-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Thymidylate synthase antibody (15047-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

Thymidylate synthase Polyclonal antibody

Thymidylate synthase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15047-1-AP
Publications(44)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

TYMS, DKCD, EC:2.1.1.45, HsT422, OK/SW-cl.29

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, HEK-293 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15047-1-AP targets Thymidylate synthase in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7027

Product name: Recombinant human TYMS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-313 aa of BC002567

Sequence: MPVAGSELPRRPLPPAAQERDAEPRPPHGELQYLGQIQHILRCGVRKDDRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIKGSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV

相同性解析による交差性が予測される生物種
Full Name thymidylate synthetase
Calculated molecular weight 36 kDa
Observed molecular weight 32-36 kDa
GenBank accession numberBC002567
Gene Symbol TYMS
Gene ID (NCBI) 7298
RRIDAB_2210721
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP04818
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymidylate synthase gene (TYMS) encodes thymidylate synthase (TS) which is an important factor in the growth of tumor cells. TS can catalyze the transformation of intracellular uridine monophosphate (dump) into dTMP which is required for DNA replication and repairing. TS is a key enzyme in the process of cell proliferation, also is the important target enzymes of 5-FU and other chemotherapy drugs. The expression of TYMS is negatively correlated with the efficacy of chemotherapy and the prognosis of patients who suffer from rectal cancer, breast cancer, colorectal cancer, gastric cancer, head and neck cancer, esophageal cancer, pancreatic cancer and so on.

Protocols

Product Specific Protocols
IF protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
IHC protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
IP protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
WB protocol for Thymidylate synthase antibody 15047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Metab

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions

Authors - Owen S Skinner
  • KO Validated
View Article
humanWB

Cancer Res

5-Fluorouracil Enhances the Antitumor Activity of the Glutaminase Inhibitor CB-839 against PIK3CA-Mutant Colorectal Cancers.

Authors - Yiqing Zhao
View Article

Nat Commun

CETSA screening identifies known and novel thymidylate synthase inhibitors and slow intracellular activation of 5-fluorouracil.

Authors - Helena Almqvist
View Article
humanWB

Cell Discov

Proteogenomic insights into the biology and treatment of pan-melanoma

Authors - Hang Xiang
View Article
humanWB

Cell Discov

Lysine butyrylation of HSP90 regulated by KAT8 and HDAC11 confers chemoresistance

Authors - Yan He
View Article

Redox Biol

Redistribution of defective mitochondria-mediated dihydroorotate dehydrogenase imparts 5-fluorouracil resistance in colorectal cancer

Authors - Shuohui Dong
View Article
||
New chat

Able™

正在加载，请稍候...