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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TYK2 Recombinant antibody (83161-5-RR)

    IF Staining of HepG2 using 83161-5-RR (same clone as 83161-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (83161-5-RR, Clone: 230176B6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83161-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using TYK2 Recombinant antibody (83161-5-RR)

    FC experiment of U2OS using 83161-5-RR (same clone as 83161-5-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug Tyk2 Recombinant Antibody (83161-5-RR, Clone:230176B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83161-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83161-5-RR

    Affinity and Kinetic Characterization of 83161-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83161-5-RR against Human TYK2 were performed. The affinity constant is 0.153 nM.

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TYK2 Recombinant antibody, PBS Only

TYK2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83161-5-PBS
Clone No.230176B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

tyrosine kinase 2, JTK1, EC:2.7.10.2, 230176B6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83161-5-PBS targets TYK2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TYK2 fusion protein Ag28457 相同性解析による交差性が予測される生物種
Full Name tyrosine kinase 2
Calculated molecular weight 1187 aa, 134 kDa
GenBank accession numberBC014243
Gene Symbol TYK2
Gene ID (NCBI) 7297
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP29597
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.