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  • Featured Product
  • KD/KO Validated

TXNL4A Polyclonal antibody

TXNL4A Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 27646-1-AP

Synonyms

DIB1, DIM1, DIM1 protein homolog, HsT161, thioredoxin like 4A, Thioredoxin like protein 4A, TXNL4, TXNL4A, U5 15kD

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 27646-1-AP

    WB analysis of HEK-293 using 27646-1-AP

    WB result of TXNL4A antibody (27646-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TXNL4A transfected HEK-293 cells.

  • WB analysis using 27646-1-AP

    WB analysis using 27646-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27646-1-AP (TXNL4A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 27646-1-AP

    WB analysis of PC-3 using 27646-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 27646-1-AP (TXNL4A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 27646-1-AP

    IF Staining of HepG2 using 27646-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 27646-1-AP (TXNL4A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, PC-3 cells, L02 cells, HepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

27646-1-AP targets TXNL4A in WB, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNL4A fusion protein Ag26473 相同性解析による交差性が予測される生物種
Full Name thioredoxin-like 4A
Calculated molecular weight 142 aa, 17 kDa
Observed molecular weight 14-20 kDa
GenBank accession numberBC001046
Gene symbol TXNL4A
Gene ID (NCBI) 10907
RRIDAB_2880937
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TXNL4A antibody 27646-1-APDownload protocol
IF protocol for TXNL4A antibody 27646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Struct Mol Biol

Structure of the human 20S U5 snRNP

Authors - Sarah Schneider
View Article