Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 25465-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 25465-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 25465-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 25465-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 25465-1-AP (TWIST1-specific antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 25465-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 25465-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 25465-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 25465-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 25465-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 25465-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25465-1-AP (TWIST1-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of L02 using 25465-1-AP
Immunofluorescent analysis of (4% PFA) fixed L02 cells using TWIST1-specific antibody (25465-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Featured Product
KD/KO Validated

TWIST1-specific Polyclonal antibody

TWIST1-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25465-1-AP

Publications(251)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, CoIP, ChIP, ELISA

Synonyms

TWIST1, ACS3, bHLHa38, BPES2, BPES3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (251)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	U-251 cells, Raji cells, A549 cells, SH-SY5Y cells, MDA-MB-231 cells, PC-3 cells
Positive IHC detected in	human liver cancer tissue, human breast cancer tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 11 publications below
WB	See 219 publications below
IHC	See 39 publications below
IF	See 15 publications below
CoIP	See 4 publications below
ChIP	See 8 publications below

Product Information

25465-1-AP targets TWIST1-specific in WB, IHC, IF/ICC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, pig, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21924 Product name: Recombinant human TWIST1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 12-72 aa of BC036704 Sequence: PADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQG 相同性解析による交差性が予測される生物種
Full Name	twist homolog 1 (Drosophila)
Calculated molecular weight	202 aa, 21 kDa
Observed molecular weight	25-30 kDa
GenBank accession number	BC036704
Gene Symbol	TWIST1
Gene ID (NCBI)	7291
RRID	AB_2880093
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15672
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Twist-related protein 1 (TWIST1), also named as BHLHA38, is 202 amino acid protein, which contains one bHLH domain. TWIST1 localizes in the nucleus and acts as a transcriptional regulator. TWIST1 inhibits monogenesis by sequestrating E proteins and inhibits tans-activation by MEF2. TWIST1 is expressed in subset of mesodermal cells. The antibody reacts specifically with Twist1 protein. The calculated molecular weight of TWIST1 is 21 kDa, but the observed molecular weight is about 30 kDa due to the post-translational modification(PMID: 30733340).

Protocols

Product Specific Protocols
IF protocol for TWIST1-specific antibody 25465-1-AP	Download protocol
IHC protocol for TWIST1-specific antibody 25465-1-AP	Download protocol
WB protocol for TWIST1-specific antibody 25465-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther Focal adhesion kinase/Src family kinase axis-mediated tyrosine phosphorylation of metabolic enzymes facilitates tumor metastasis Authors - Jie Chen View Article
human	WB	Signal Transduct Target Ther MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma. Authors - Qin Tang View Article
human	WB	J Hematol Oncol Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy. Authors - Tong Shen View Article
human	WB	Mol Cancer GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis Authors - Linfei Huang View Article
human	WB,IHC	Cell Metab ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition. Authors - Ming Lu KD Validated View Article
human,mouse	WB	Clin Mol Hepatol MET promotes hepatocellular carcinoma development through the promotion of TRIB3-mediated FOXO1 degradation Authors - Tiantian Wang View Article

Validation Data Gallery

WB analysis using 25465-1-AP

WB analysis of SH-SY5Y using 25465-1-AP

WB analysis of A549 using 25465-1-AP

WB analysis of Raji using 25465-1-AP

IHC staining of human pancreas cancer using 25465-1-AP

IHC staining of human liver cancer using 25465-1-AP

IHC staining of human breast cancer using 25465-1-AP

IHC staining of human breast cancer using 25465-1-AP

IHC staining of human pancreas cancer using 25465-1-AP

IHC staining of human liver cancer using 25465-1-AP

IF Staining of L02 using 25465-1-AP

TWIST1-specific Polyclonal antibody

TWIST1-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Focal adhesion kinase/Src family kinase axis-mediated tyrosine phosphorylation of metabolic enzymes facilitates tumor metastasis

MELK is an oncogenic kinase essential for metastasis, mitotic progression, and programmed death in lung carcinoma.

Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy.

GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis

ACOT12-Dependent Alteration of Acetyl-CoA Drives Hepatocellular Carcinoma Metastasis by Epigenetic Induction of Epithelial-Mesenchymal Transition.

MET promotes hepatocellular carcinoma development through the promotion of TRIB3-mediated FOXO1 degradation

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