Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, MCF-7 cells, SGC-7901 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 17 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
18125-1-AP targets TWIST1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12700 Product name: Recombinant human TWIST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-202 aa of BC036704 Sequence: MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH 相同性解析による交差性が予測される生物種 |
| Full Name | twist homolog 1 (Drosophila) |
| Calculated molecular weight | 202 aa, 21 kDa |
| Observed molecular weight | 22 kDa, 28 kDa, 45-60 kDa |
| GenBank accession number | BC036704 |
| Gene Symbol | TWIST1 |
| Gene ID (NCBI) | 7291 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15672 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TWIST1, a basic helix-loop-helix protein which acts as a transcriptional regulator, plays a central role in differentiation of mesodermal during embryogenesis. It is also implicated in tumorigenesis, invasion and metastasis. TWIST1 forms both homodimers(45KD) and heterodimers with E2A E proteins, and dimer partner selection is a critical mediator of TWIST function. (PMID:14724576, 16502419).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TWIST1 antibody 18125-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis. | ||
Int J Biochem Cell Biol Blue light emitting diodes irradiation causes cell death in colorectal cancer by inducing ROS production and DNA damage. | ||
Cancer Lett Downregulation of ACE2/Ang-(1-7)/Mas axis promotes breast cancer metastasis by enhancing store-operated calcium entry. | ||
Int J Cancer Down-regulation of KIAA1199/CEMIP by miR-216a suppresses tumor invasion and metastasis in colorectal cancer. | ||
Sci Rep Conditional ablation of TGF-β signaling inhibits tumor progression and invasion in an induced mouse bladder cancer model. |
