Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HEK-293 cells, HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14769-1-AP targets TWF2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6553 Product name: Recombinant human TWF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC000327 Sequence: MAHQTGIHATEELKEFFAKARAGSVRLIKVVIEDEQLVLGASQEPVGRWDQDYDRAVLPLLDAQQPCYLLYRLDSQNAQGFEWLFLAWSPDNSPVRLKMLYAATRATVKKEFGGGHIKDELFGTVKDDLSFAGYQKHLSSCAAPAPLTSAERELQQIRINEVKTEISVESKHQTLQGLAFPLQPEAQRALQQLKQKMVNYIQMKLDLERETIELVHTEPTDVAQLPSRVPRDAARYHFFLYKHTHEGDPLESVVFIYSMPGYKCSIKERMLYSSCKSRLLDSVEQDFHLEIAKKIEIGDGAELTAEFLYDEVHPKQHAFKQAFAKPKGPGGKRGHKRLIRGPGENGDDS 相同性解析による交差性が予測される生物種 |
| Full Name | twinfilin, actin-binding protein, homolog 2 (Drosophila) |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC000327 |
| Gene Symbol | TWF2 |
| Gene ID (NCBI) | 11344 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IBS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TWF2, also known as PTK9L and A6RP, contains a consensus sequence for actin-depolymerizing proteins and 2 ATP-binding motifs. TWF2 is an actin-binding protein that inhibits actin polymerization by sequestering G-actin. TWF2 plays an important role in clathrin-mediated endocytosis and distribution of endocytic organelles (PMID: 10406962).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TWF2 antibody 14769-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |