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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TWF1 Monoclonal antibody (67796-1-Ig)

    WB analysis using 67796-1-Ig (same clone as 67796-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67796-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using TWF1 Monoclonal antibody (67796-1-Ig)

    WB analysis of Jurkat using 67796-1-Ig (same clone as 67796-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67796-1-Ig (TWF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67796-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TWF1 Monoclonal antibody (67796-1-Ig)

    IF Staining of MCF-7 using 67796-1-Ig (same clone as 67796-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TWF1 antibody (67796-1-Ig, Clone: 2F10F11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67796-1-PBS in a different storage buffer formulation.

TWF1 Monoclonal antibody, PBS Only

TWF1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67796-1-PBS
Clone No.2F10F11

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

A6, Protein A6, Protein tyrosine kinase 9, PTK9, TWF1, Twinfilin 1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67796-1-PBS targets TWF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag28630

Product name: Recombinant human TWF1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-342 aa of BC022344

Sequence: ASEDVKEIFARARNGKYRLLKISIENEQLVIGSYSQPSDSWDKDYDSFVLPLLEDKQPCYILFRLDSQNAQGYEWIFIAWSPDHSHVRQKMLYAATRATLKKEFGGGHIKDEVFGTVKEDVSLHGYKKYLLSQSSPAPLTAAEEELRQIKINEVQTDVGVDTKHQTLQGVAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSFAKPKGPAGKRGIRRLIRGPAETEATTD

相同性解析による交差性が予測される生物種
Full Name twinfilin, actin-binding protein, homolog 1 (Drosophila)
Calculated molecular weight 368 aa, 42 kDa
Observed molecular weight36-38 kDa
GenBank accession numberBC022344
Gene Symbol TWF1
Gene ID (NCBI) 5756
RRIDAB_2918560
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ12792
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Twinfilin-1 (TWF1) is an actin-binding protein implicated in cell motility, invasion and migration. Its expression is linked to sensitivity to drugs and cancer progression. Overexpression of TWF1 has been reported in various cancers, including breast cancer and lung cancer.

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