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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using TULP1 Polyclonal antibody (18971-1-AP)

    WB analysis of human brain using 18971-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TULP1 Polyclonal antibody (18971-1-AP)

    WB analysis using 18971-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using TULP1 Polyclonal antibody (18971-1-AP)

    WB analysis of human brain using 18971-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18971-1-AP (TULP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human eye tissue using TULP1 Polyclonal antibody (18971-1-AP)

    IHC staining of human eye using 18971-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human eye tissue using TULP1 Polyclonal antibody (18971-1-AP)

    IHC staining of human eye using 18971-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 18971-1-AP (TULP1 antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

TULP1 Polyclonal antibody

TULP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18971-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TUBL1, Tubby-related protein 1, Tubby-like protein 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inY79 cells, human brain tissue, mouse retina tissue
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18971-1-AP targets TULP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TULP1 fusion protein Ag5598 相同性解析による交差性が予測される生物種
Full Name tubby like protein 1
Calculated molecular weight 489 aa, 55 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC032714
Gene Symbol TULP1
Gene ID (NCBI) 7287
RRIDAB_2211412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00294
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TULP1, also known as RP14, LCA15, and TUBL1, is a member of the tubby-like protein (TULP) family. TULP1 is expressed exclusively in the retina and is essential for the transport of rhodopsin from its site of synthesis in the inner segments through the connecting cilium to the outer segments(PMID: 17620573). Mutations in TULP1 are associated with Retinitis pigmentosa 14 and Leber congenital amaurosis-15(PMID: 17620573, 17962469).

Protocols

Product Specific Protocols
WB protocol for TULP1 antibody 18971-1-APDownload protocol
IHC protocol for TULP1 antibody 18971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Sci Rep

Proteomics identifies multiple retinitis pigmentosa associated proteins involved in retinal degeneration in a mouse model bearing a Pde6b mutation

Authors - Mingzhu Yang
View Article