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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TUFM Monoclonal antibody (67802-1-Ig)

    WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using TUFM Monoclonal antibody (67802-1-Ig)

    WB analysis using 67802-1-Ig (same clone as 67802-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67802-1-Ig (TUFM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using TUFM Monoclonal antibody (67802-1-Ig)

    IP experiment of HepG2 using 67802-1-Ig (same clone as 67802-1-PBS)

    IP result of anti-TUFM (IP:67802-1-Ig, 4ug; Detection:67802-1-Ig 1:3000) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TUFM Monoclonal antibody (67802-1-Ig)

    IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TUFM Monoclonal antibody (67802-1-Ig)

    IHC staining of human colon cancer using 67802-1-Ig (same clone as 67802-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67802-1-Ig (TUFM antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67802-1-PBS in a different storage buffer formulation.

TUFM Monoclonal antibody, PBS Only

TUFM Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 67802-1-PBS
Clone No.2A10D2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67802-1-PBS targets TUFM in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag24976

Product name: Recombinant human TUFM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 108-455 aa of BC010041

Sequence: GITINAAHVEYSTAARHYAHTDCPGHADYVKNMITGTAPLDGCILVVAANDGPMPQTREHLLLARQIGVEHVVVYVNKADAVQDSEMVELVELEIRELLTEFGYKGEETPVIVGSALCALEGRDPELGLKSVQKLLDAVDTYIPVPARDLEKPFLLPVEAVYSVPGRGTVVTGTLERGILKKGDECELLGHSKNIRTVVTGIEMFHKSLERAEAGDNLGALVRGLKREDLRRGLVMVKPGSIKPHQKVEAQVYILSKEEGGRHKPFVSHFMPVMFSLTWDMACRIILPPEKELAMPGEDLKFNLILRQPMILEKGQRFTLRDGNRTIGTGLVTNTLAMTEEEKNIKWG

相同性解析による交差性が予測される生物種
Full Name Tu translation elongation factor, mitochondrial
Calculated molecular weight 50 kDa
Observed molecular weight48-50 kDa
GenBank accession numberBC010041
Gene Symbol TUFM
Gene ID (NCBI) 7284
RRIDAB_2918565
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP49411
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

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