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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TUBGCP2 Polyclonal antibody (25856-1-AP)

    WB analysis using 25856-1-AP (same clone as 25856-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25856-1-AP (TUBGCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25856-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using TUBGCP2 Polyclonal antibody (25856-1-AP)

    IP experiment of HeLa using 25856-1-AP (same clone as 25856-1-PBS)

    IP result of anti-TUBGCP2 (IP:25856-1-AP, 4ug; Detection:25856-1-AP 1:500) with HeLa cells lysate 3920 ug. This data was developed using the same antibody clone with 25856-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TUBGCP2 Polyclonal antibody (25856-1-AP)

    IHC staining of mouse testis using 25856-1-AP (same clone as 25856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25856-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TUBGCP2 Polyclonal antibody (25856-1-AP)

    IHC staining of mouse testis using 25856-1-AP (same clone as 25856-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25856-1-PBS in a different storage buffer formulation.

TUBGCP2 Polyclonal antibody, PBS Only

TUBGCP2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 25856-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25856-1-PBS targets TUBGCP2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23066

Product name: Recombinant human TUBGCP2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 553-902 aa of BC093770

Sequence: ALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVTAQ

相同性解析による交差性が予測される生物種
Full Name tubulin, gamma complex associated protein 2
Calculated molecular weight 902 aa, 103 kDa
Observed molecular weight 103 kDa
GenBank accession numberBC093770
Gene Symbol TUBGCP2
Gene ID (NCBI) 10844
RRIDAB_2880271
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSJ2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TUBGCP2 (tubulin-gamma-complex-associated protein 2) s TUBGCP2 is a core component of the g-TuRC nucleation complex (PMID: 3345861). Pathogenic variants in TUBGCP2 cause an autosomal recessive neurodevelopmental trait consisting of a neuronal migration disord (PMID: 31630790).

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