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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TUBG2 Polyclonal antibody

TUBG2 Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28009-1-AP

Synonyms

Gamma 2 tubulin, GTU-88, TUBG2, Tubulin gamma 2 chain, tubulin, gamma 2

Validation Data Gallery

Applications:
  • WB analysis using 28009-1-AP

    WB analysis using 28009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28009-1-AP

    WB analysis using 28009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28009-1-AP (TUBG2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 28009-1-AP

    IHC staining of human lung cancer using 28009-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28009-1-AP (TUBG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HepG2 using 28009-1-AP

    FC experiment of HepG2 using 28009-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human TUBG2 (28009-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse cerebellum tissue, HeLa cells, T-47D cells, rat cerebellum tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

28009-1-AP targets TUBG2 in WB, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TUBG2 fusion protein Ag27728 相同性解析による交差性が予測される生物種
Full Name tubulin, gamma 2
Calculated molecular weight 451 aa, 51 kDa
Observed molecular weight51 kDa
GenBank accession numberBC051890
Gene symbol TUBG2
Gene ID (NCBI) 27175
RRIDAB_2918138
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TUBG2 antibody 28009-1-APDownload protocol
IHC protocol for TUBG2 antibody 28009-1-APDownload protocol
FC protocol for TUBG2 antibody 28009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols