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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTYH3 Polyclonal antibody (24707-1-AP)

    WB analysis using 24707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24707-1-AP (TTYH3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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TTYH3 Polyclonal antibody

TTYH3 Polyclonal Antibody for WB, ELISA
Cat No. 24707-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

hTTY3, KIAA1691, Protein tweety homolog 3, TTYH3, tweety homolog 3 (Drosophila)

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24707-1-AP targets TTYH3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTYH3 fusion protein Ag18804 相同性解析による交差性が予測される生物種
Full Name tweety homolog 3 (Drosophila)
Calculated molecular weight 523 aa, 58 kDa
Observed molecular weight50 kDa
GenBank accession numberBC131824
Gene Symbol TTYH3
Gene ID (NCBI) 80727
RRIDAB_3085749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C0H2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tweety homologs (TTYHs) are conserved transmembrane proteins present in all eukaryotes including three members (TTYH1, TTYH2, and TTYH3) in humans. They are widely expressed in mammals including at high levels in the nervous system and have been implicated in cancers and other diseases including epilepsy, chronic pain, and viral infections. Physiologically, TTYH2 and TTYH3 are broadly expressed, while expression of TTYH1 is primarily limited to the nervous system, testes, and stem cells. TTYH3 is upregulated in gastric cancer with higher expression correlated with poor clinical outcomes. (PMID: 34824283)

Protocols

Product Specific Protocols
WB protocol for TTYH3 antibody 24707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols