Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human gliomas Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
26973-1-AP targets TTYH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25290 Product name: Recombinant human TTYH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 264-390 aa of BC011347 Sequence: EAATAVGLSDFCSNPDPYVLNLTQEETGLSSDILSYYLLCNRAVSNPFQQRLTLSQRALANIHSQLLGLEREAVPQFPSAQKPLLSLEETLNVTEGNFHQLVALLHCRSLHKDYGAALRGLCEDALE 相同性解析による交差性が予測される生物種 |
| Full Name | tweety homolog 1 (Drosophila) |
| Calculated molecular weight | 49-50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC011347 |
| Gene Symbol | TTYH1 |
| Gene ID (NCBI) | 57348 |
| RRID | AB_2880707 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H313 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TTYH1, protein tweety homolog 1, is a glycosylated protein composed of five predicted transmembrane segments, which belongs to the tweety family. The expression of TTYH1 is restricted mainly to neural tissue and is up-regulated in astrocytoma, glioma and several other cancers. TTYH1 has some isoforms with MW of 49-50 kDa, 35 kDa and 26 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TTYH1 antibody 26973-1-AP | Download protocol |
| WB protocol for TTYH1 antibody 26973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cancer Elevated expression of Tweety homologue 3 predicts poor clinical outcomes in ovarian cancer. | ||
Nat Commun Endolysosomal processing of neuron-derived signaling lipids regulates autophagy and lipid droplet degradation in astrocytes |
