×
Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using TTYH1 Polyclonal antibody (26973-1-AP)

    WB analysis using 26973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of pig brain tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IP experiment of pig brain using 26973-1-AP

    IP result of anti-TTYH1 (IP:26973-1-AP, 4ug; Detection:26973-1-AP 1:1000) with pig brain tissue lysate 1240 ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IHC staining of human gliomas using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IHC staining of human gliomas using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IHC staining of mouse brain using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IHC staining of mouse brain using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IHC staining of human ovary cancer using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IHC staining of mouse cerebellum using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using TTYH1 Polyclonal antibody (26973-1-AP)

    IHC staining of mouse testis using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

TTYH1 Polyclonal antibody

TTYH1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26973-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

hTTY1, Protein tweety homolog 1, tweety homolog 1 (Drosophila), Volume-regulated anion channel subunit TTYH1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inpig brain tissue
Positive IHC detected inhuman gliomas, mouse testis tissue, human ovary cancer tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26973-1-AP targets TTYH1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag25290

Product name: Recombinant human TTYH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 264-390 aa of BC011347

Sequence: EAATAVGLSDFCSNPDPYVLNLTQEETGLSSDILSYYLLCNRAVSNPFQQRLTLSQRALANIHSQLLGLEREAVPQFPSAQKPLLSLEETLNVTEGNFHQLVALLHCRSLHKDYGAALRGLCEDALE

相同性解析による交差性が予測される生物種
Full Name tweety homolog 1 (Drosophila)
Calculated molecular weight 49-50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC011347
Gene Symbol TTYH1
Gene ID (NCBI) 57348
RRIDAB_2880707
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTYH1, protein tweety homolog 1, is a glycosylated protein composed of five predicted transmembrane segments, which belongs to the tweety family. The expression of TTYH1 is restricted mainly to neural tissue and is up-regulated in astrocytoma, glioma and several other cancers. TTYH1 has some isoforms with MW of 49-50 kDa, 35 kDa and 26 kDa.

Protocols

Product Specific Protocols
IHC protocol for TTYH1 antibody 26973-1-APDownload protocol
IP protocol for TTYH1 antibody 26973-1-APDownload protocol
WB protocol for TTYH1 antibody 26973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nat Commun

Endolysosomal processing of neuron-derived signaling lipids regulates autophagy and lipid droplet degradation in astrocytes

Authors - Jagannatham Naidu Bhupana
View Article
humanWB,IHC

J Cancer

Elevated expression of Tweety homologue 3 predicts poor clinical outcomes in ovarian cancer.

Authors - Junhan Zhou
View Article
||
New chat

Able™

正在加载，请稍候...