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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TTPA Monoclonal antibody

TTPA Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E7D10

Cat no : 67431-1-Ig

Synonyms

Alpha TTP, alphaTTP, ATTP, AVED, TPP1, TTP1, TTPA

Validation Data Gallery

Applications:
  • WB analysis of rat liver using 67431-1-Ig

    WB analysis of rat liver using 67431-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 67431-1-Ig

    WB analysis of rat liver using 67431-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig liver using 67431-1-Ig

    WB analysis of pig liver using 67431-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 67431-1-Ig

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTPA/TPP1 antibody included
  • IHC staining of human liver cancer using 67431-1-Ig

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTPA/TPP1 antibody included
  • IF Staining of human liver cancer using 67431-1-Ig

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 67431-1-Ig

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inrat liver tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy TTPA/TPP1 Ready-To-Use IHC Kit. TTPA/TPP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67431-1-Ig targets TTPA in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TTPA fusion protein Ag13587 相同性解析による交差性が予測される生物種
Full Name tocopherol (alpha) transfer protein
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC058000
Gene symbol TTPA
Gene ID (NCBI) 7274
RRIDAB_2882669
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TTPA antibody 67431-1-IgDownload protocol
IHC protocol for TTPA antibody 67431-1-IgDownload protocol
IF protocol for TTPA antibody 67431-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols