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Validation Data Gallery

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  • Western Blot (WB) analysis of rat liver tissue using TTPA Monoclonal antibody (67431-1-Ig)

    WB analysis of rat liver using 67431-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using TTPA Monoclonal antibody (67431-1-Ig)

    WB analysis of rat liver using 67431-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using TTPA Monoclonal antibody (67431-1-Ig)

    WB analysis of pig liver using 67431-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using TTPA Monoclonal antibody (67431-1-Ig)

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

TTPA Monoclonal antibody

TTPA Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67431-1-Ig
Clone No.3E7D10

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

Alpha TTP, alphaTTP, ATTP, AVED, TPP1, TTP1, TTPA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat liver tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67431-1-Ig targets TTPA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Reactivity Human, Mouse, Rat, Pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag13587

Product name: Recombinant human TTPA protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-278 aa of BC058000

Sequence: MAEARSQPSAGPQLNALPDHSPLLQPGLAALRRRAREAGVPLAPLPLTDSFLLRFLRARDFDLDLAWRLLKNYYKWRAECPEISADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWDPKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDLEGWQFSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQSLLQHFPDILPLEYGGEEFSMEDICQEWTNFIMKSEDYLSSISESIQ

相同性解析による交差性が予測される生物種
Full Name tocopherol (alpha) transfer protein
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC058000
Gene Symbol TTPA
Gene ID (NCBI) 7274
RRIDAB_2882669
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP49638
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TTPA antibody 67431-1-IgDownload protocol
IHC protocol for TTPA antibody 67431-1-IgDownload protocol
IF protocol for TTPA antibody 67431-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols