Validation Data Gallery
Tested Applications
| Positive WB detected in | rat liver tissue, pig liver tissue | 
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0  | 
| Positive IF-P detected in | human liver cancer tissue | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 | 
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67431-1-Ig targets TTPA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Reactivity | Human, Mouse, Rat, Pig | 
| Host / Isotype | Mouse / IgG2b | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | 
                                             CatNo: Ag13587 Product name: Recombinant human TTPA protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-278 aa of BC058000 Sequence: MAEARSQPSAGPQLNALPDHSPLLQPGLAALRRRAREAGVPLAPLPLTDSFLLRFLRARDFDLDLAWRLLKNYYKWRAECPEISADLHPRSIIGLLKAGYHGVLRSRDPTGSKVLIYRIAHWDPKVFTAYDVFRVSLITSELIVQEVETQRNGIKAIFDLEGWQFSHAFQITPSVAKKIAAVLTDSFPLKVRGIHLINEPVIFHAVFSMIKPFLTEKIKERIHMHGNNYKQSLLQHFPDILPLEYGGEEFSMEDICQEWTNFIMKSEDYLSSISESIQ 相同性解析による交差性が予測される生物種 | 
                                    
| Full Name | tocopherol (alpha) transfer protein | 
| Calculated molecular weight | 32 kDa | 
| Observed molecular weight | 32 kDa | 
| GenBank accession number | BC058000 | 
| Gene Symbol | TTPA | 
| Gene ID (NCBI) | 7274 | 
| RRID | AB_2882669 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Protein A purification | 
| UNIPROT ID | P49638 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TTPA antibody 67431-1-Ig | Download protocol | 
| IHC protocol for TTPA antibody 67431-1-Ig | Download protocol | 
| WB protocol for TTPA antibody 67431-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 






