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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using TTDN1 Polyclonal antibody (25410-1-AP)

    WB analysis of NIH/3T3 using 25410-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25410-1-AP (TTDN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using TTDN1 Polyclonal antibody (25410-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 25410-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 25410-1-AP (TTDN1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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TTDN1 Polyclonal antibody

TTDN1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25410-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C7orf11, ABHS, TTD non-photosensitive 1 protein, ORF20, MPLKIP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25410-1-AP targets TTDN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTDN1 fusion protein Ag22094 相同性解析による交差性が予測される生物種
Full Name chromosome 7 open reading frame 11
Calculated molecular weight 179 aa, 19 kDa
Observed molecular weight23 kDa
GenBank accession numberBC026265
Gene Symbol TTDN1
Gene ID (NCBI) 136647
RRIDAB_2935461
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAP9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TTDN1 colocalizes with Plk1 at the centrosome in mitosis and the midbody during cytokinesis. It is phosphorylated by Cdk1 in mitosis, and this is required for its interaction with Plk1. Overexpression of TTDN1 or its knockdown by siRNA causes multi-polar spindles and multiple nuclei, suggesting that TTDN1 plays a role in regulating mitosis and cytokinesis. Mutations in the TTDN1 gene are associated with a distinct trichothiodystrophy phenotype. (PMID:17310276, 25290684)

Protocols

Product Specific Protocols
WB protocol for TTDN1 antibody 25410-1-APDownload protocol
IHC protocol for TTDN1 antibody 25410-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols