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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using TTC39A Polyclonal antibody (21323-1-AP)

    WB analysis of mouse liver using 21323-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21323-1-AP (TTC39A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TTC39A Polyclonal antibody (21323-1-AP)

    WB analysis of MCF-7 using 21323-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 21323-1-AP (TTC39A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TTC39A Polyclonal antibody (21323-1-AP)

    IHC staining of human breast cancer using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TTC39A Polyclonal antibody (21323-1-AP)

    IHC staining of human breast cancer using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TTC39A Polyclonal antibody (21323-1-AP)

    IHC staining of human testis using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TTC39A Polyclonal antibody (21323-1-AP)

    IHC staining of human testis using 21323-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21323-1-AP (TTC39A antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using TTC39A Polyclonal antibody (21323-1-AP)

    IF Staining of HepG2 using 21323-1-AP

    Immunofluorescent analysis of HepG2 cells, using TTC39A antibody 21323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TTC39A Polyclonal antibody (21323-1-AP)

    IF Staining of HeLa using 21323-1-AP

    Immunofluorescent analysis of Hela cells, using TTC39A antibody 21323-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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TTC39A Polyclonal antibody

TTC39A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21323-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C1orf34, DEME 6, KIAA0452, TPR repeat protein 39A, TTC39A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21323-1-AP targets TTC39A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC39A fusion protein Ag14874 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 39A
Calculated molecular weight 613 aa, 70 kDa
Observed molecular weight 65-70 kDa
GenBank accession numberBC004399
Gene Symbol TTC39A
Gene ID (NCBI) 22996
RRIDAB_10733645
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SRH9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TTC39A antibody 21323-1-APDownload protocol
IHC protocol for TTC39A antibody 21323-1-APDownload protocol
IF protocol for TTC39A antibody 21323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols