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Species Reactivity

Species Reactivity

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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    WB analysis of PC-3 using 21693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    WB analysis of PC-3 using 21693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    WB analysis of PC-3 using 21693-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 21693-1-AP (TTC31 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human testis using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human testis using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human liver using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TTC31 Polyclonal antibody (21693-1-AP)

    IHC staining of human liver using 21693-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 21693-1-AP (TTC31 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using TTC31 Polyclonal antibody (21693-1-AP)

    IF Staining of PC-3 using 21693-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 21693-1-AP (TTC31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

TTC31 Polyclonal antibody

TTC31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21693-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

TPR repeat protein 31

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inPC-3 cells
Positive IHC detected inhuman testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21693-1-AP targets TTC31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16281

Product name: Recombinant human TTC31 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-358 aa of BC098365

Sequence: MAPIPKTVGRIKLDCSLRPSCPLEVAAAPKLCKEFGPEDYGEEDIVDFLRRLVESDPQGLHRIHVDGSSGRLQLWHHDYLLGHLDDEGKSTGQSDRGKGAEGLGTYCGLRKSFLYPPQESEPCPQSPSASATFPSVSDSLLQVAMPQKLLVTEEEANRLAEELVAEEERMKQKAEKKRLKKKRQKERKRQERLEQYCGEPKASTTSDGDESPPSSPGNPVQGQCGEEEDSLDLSSTFVSLALRKVGDWPLSARREKGLNQEPQGRGLALQKMGQEEESPPREERPQQSPKEKDLGRLRPQDLLDFAPYPQASPGLLAAALQQSQELAKLGTSFAQNGFYHEAVVLFTQALKLNPQDHR

相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 31
Calculated molecular weight 358 aa, 40 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC098365
Gene Symbol TTC31
Gene ID (NCBI) 64427
RRIDAB_11182824
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ49AM3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for TTC31 antibody 21693-1-APDownload protocol
IHC protocol for TTC31 antibody 21693-1-APDownload protocol
WB protocol for TTC31 antibody 21693-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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