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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC30A Monoclonal antibody (68232-1-Ig)

    WB analysis using 68232-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using TTC30A Monoclonal antibody (68232-1-Ig)

    WB analysis using 68232-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68232-1-Ig (TTC30A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TTC30A Monoclonal antibody (68232-1-Ig)

    IF Staining of hTERT-RPE1 using 68232-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTC30A antibody (68232-1-Ig, Clone: 1A11B3 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, green).

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TTC30A Monoclonal antibody

TTC30A Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68232-1-Ig
Clone No.1A11B3

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ13946, FLJ77601, TPR repeat protein 30A, TTC30A

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, rat testis tissue, HeLa cells, HEK-293 cells, mouse testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68232-1-Ig targets TTC30A in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TTC30A fusion protein Ag18361 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 30A
Calculated molecular weight 665 aa, 76 kDa
Observed molecular weight 72-76 kDa
GenBank accession numberBC042848
Gene Symbol TTC30A
Gene ID (NCBI) 92104
RRIDAB_2935319
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ86WT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tetratricopeptide repeat protein 30A (TTC30A) as a component of ciliary segmentation, may be essential for cartilage differentiation and renal tubulogenesis (PMID: 34548398). It is required for polyglutamylation of axonemal tubulin and plays a role in anterograde intraflagellar transport (IFT), the process by which cilia precursors are transported from the base of the cilium to the site of their incorporation at the tip.

Protocols

Product Specific Protocols
WB protocol for TTC30A antibody 68232-1-IgDownload protocol
IF protocol for TTC30A antibody 68232-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols