Validation Data Gallery
Tested Applications
| Positive IP detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25354-1-AP targets TTC25 in IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18604 Product name: Recombinant human TTC25 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-344 aa of BC067297 Sequence: MSDPEGETLRSTFPSYMAEGERLYLCGEFSKAAQSFSNALYLQDGDKNCLVARSKCFLKMGDLERSLKDAEASLQSDPAFCKGILQKAETLYTMGDFEFALVFYHRGYKLRPDREFRVGIQKAQEAINNSVGSPSSIKLENKGDLSFLSKQAENIKAQQKPQPMKHLLHPTKGEPKWKASLKSEKTVRQLLGELYVDKEYLEKLLLDEDLIKGTMKGGLTVEDLIMTGINYLDTHSNFWRQQKPIYARERDRKLMQEKWLRDHKRRPSQTAHYILKSLEDIDMLLTSGSAEGSLQKAEKVLKKVLEWNKEEVPNKDELVGNLYSCIGNAQIELGQMEAALQSHR 相同性解析による交差性が予測される生物種 |
| Full Name | tetratricopeptide repeat domain 25 |
| Calculated molecular weight | 665 aa, 76 kDa |
| Observed molecular weight | 77 kDa |
| GenBank accession number | BC067297 |
| Gene Symbol | TTC25 |
| Gene ID (NCBI) | 83538 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96NG3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TTC25 is located on human chromosome 17q21.2 and comprises 12 exons. The 2.255-bp TTC25 cDNA encodes the 672-amino-acid tetratricopeptide repeat domain-containing protein 25. TTC25 contains eight tetratricopeptide (TPR) domains TTC25 plays an essential role in the correct function of motile cilia at the ventral node and is therefore important for the development of left-right body asymmetry (PMID: 27486780).