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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TTC12 Polyclonal antibody (28216-1-AP)

    WB analysis using 28216-1-AP (same clone as 28216-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28216-1-AP (TTC12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTC12 Polyclonal antibody (28216-1-AP)

    IHC staining of human lung cancer using 28216-1-AP (same clone as 28216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28216-1-AP (TTC12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28216-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using TTC12 Polyclonal antibody (28216-1-AP)

    IHC staining of human lung cancer using 28216-1-AP (same clone as 28216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28216-1-AP (TTC12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28216-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TTC12 Polyclonal antibody (28216-1-AP)

    IF Staining of A549 using 28216-1-AP (same clone as 28216-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 28216-1-AP (TTC12 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28216-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28216-1-PBS targets TTC12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TTC12 fusion protein Ag28199 相同性解析による交差性が予測される生物種
Full Name tetratricopeptide repeat domain 12
Calculated molecular weight 79 kDa
Observed molecular weight 79 kDa
GenBank accession numberBC032355
Gene Symbol TTC12
Gene ID (NCBI) 54970
RRIDAB_2881092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H892
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.