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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using TTBK2 Polyclonal antibody (15072-1-AP)

    WB analysis of PC-3 using 15072-1-AP (same clone as 15072-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using TTBK2 Polyclonal antibody (15072-1-AP)

    WB analysis of HeLa using 15072-1-AP (same clone as 15072-1-PBS)

    WB result of TTBK2 antibody (15072-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTBK2 transfected HeLa cells. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using TTBK2 Polyclonal antibody (15072-1-AP)

    WB analysis of SH-SY5Y using 15072-1-AP (same clone as 15072-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15072-1-AP (TTBK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TTBK2 Polyclonal antibody (15072-1-AP)

    IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using TTBK2 Polyclonal antibody (15072-1-AP)

    IHC staining of mouse brain using 15072-1-AP (same clone as 15072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TTBK2 Polyclonal antibody (15072-1-AP)

    IHC staining of human prostate cancer using 15072-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TTBK2 Polyclonal antibody (15072-1-AP)

    IHC staining of human prostate cancer using 15072-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TTBK2 Polyclonal antibody (15072-1-AP)

    IHC staining of human prostate cancer using 15072-1-AP (same clone as 15072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TTBK2 Polyclonal antibody (15072-1-AP)

    IHC staining of human prostate cancer using 15072-1-AP (same clone as 15072-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15072-1-AP (TTBK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using TTBK2 Polyclonal antibody (15072-1-AP)

    IF Staining of hTERT-RPE1 using 15072-1-AP (same clone as 15072-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using TTBK2 antibody (15072-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 15072-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TTBK2 Polyclonal antibody, PBS Only

TTBK2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15072-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15072-1-PBS targets TTBK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7108

Product name: Recombinant human TTBK2 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-354 aa of BC071556

Sequence: MSGGGEQPDILSVGILVKERWKVLRKIGGGGFGEIYDALDMLTRENVALKVESAQQPKQVLKMEVAVLKKLQGKDHVCRFIGCGRNDRFNYVVMQLQGRNLADLRRSQSRGTFTISTTLRLGRQILESIESIHSVGFLHRDIKPSNFAMGRFPSTCRKCYMLDFGLARQFTNSCGDVRPPRAVAGFRGTVRYASINAHRNREMGRHDDLWSLFYMLVEFVVGQLPWRKIKDKEQVGSIKERYDHRLMLKHLPPEFSIFLDHISSLDYFTKPDYQLLTSVFDNSIKTFGVIESDPFDWEKTGNDGSLTTTTTSTTPQLHTRLTPAAIGIANATPIPGDLLRENTDEVFPDEQLSD

相同性解析による交差性が予測される生物種
Full Name tau tubulin kinase 2
Calculated molecular weight 137 kDa
Observed molecular weight 137-150 kDa
GenBank accession numberBC071556
Gene Symbol TTBK2
Gene ID (NCBI) 146057
RRIDAB_2211507
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6IQ55
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TTBK2(tau tubulin kinase 2) plays an important role in the tau cascade and in spinocerebellar degeneration. The N-terminus of TTBK2 has a serine-threonine-tyrosine kinase domain, and a C-terminal region shows homology to TTBK1. TTBK1 and TTBK2 function as regulators of TDP-43 phosphorylation. TTBK1/2 may be attractive drug targets for therapeutic interventions in TDP-43 proteinopathies such as FTLD-TDP and ALS.(PMID:25473830). 15072-1-AP has a cross reaction with TTBK1.

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