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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TST Monoclonal antibody (66018-1-Ig)

    WB analysis using 66018-1-Ig (same clone as 66018-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of HEK-293 using 66018-1-Ig (same clone as 66018-1-PBS)

    WB result of TST antibody (66018-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TST transfected HEK-293 cells. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of human brain using 66018-1-Ig (same clone as 66018-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of mouse brain using 66018-1-Ig (same clone as 66018-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of mouse liver using 66018-1-Ig (same clone as 66018-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat liver tissue using TST Monoclonal antibody (66018-1-Ig)

    WB analysis of rat liver using 66018-1-Ig (same clone as 66018-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66018-1-Ig (TST Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of rat kidney using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of human liver cancer using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66018-1-Ig (TST antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using TST Monoclonal antibody (66018-1-Ig)

    IHC staining of human kidney using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66018-1-Ig(TST antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using TST Monoclonal antibody (66018-1-Ig)

    IF Staining of A549 using 66018-1-Ig (same clone as 66018-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using TST antibody (66018-1-Ig, Clone: 2A6C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66018-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

TST Monoclonal antibody, PBS Only

TST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66018-1-PBS
Clone No.2A6C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2A6C5, EC:2.8.1.1, RDS, Rhodanese

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66018-1-PBS targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag9235

Product name: Recombinant human TST protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-297 aa of BC010148

Sequence: MVHQVLYRALVSTKWLAESIRTGKLGPGLRVLDASWYSPGTREARKEYLERHVPGASFFDIEECRDTASPYEMMLPSEAGFAEYVGRLGISNHTHVVVYDGEHLGSFYAPRVWWMFRVFGHRTVSVLNGGFRNWLKEGHPVTSEPSRPEPAVFKATLDRSLLKTYEQVLENLESKRFQLVDSRSQGRFLGTEPEPDAVGLDSGHIRGAVNMPFMDFLTEDGFEKGPEELRALFQTKKVDLSQPLIATCRKGVTACHVALAAYLCGKPDVAVYDGSWSEWFRRAPPESRVSQGKSEKA

相同性解析による交差性が予測される生物種
Full Name thiosulfate sulfurtransferase (rhodanese)
Calculated molecular weight 297 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC010148
Gene Symbol TST
Gene ID (NCBI) 7263
RRIDAB_11045660
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ16762
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.

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