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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TSSK2 Polyclonal antibody (13806-1-AP)

    WB analysis using 13806-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13806-1-AP (TSSK2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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TSSK2 Polyclonal antibody

TSSK2 Polyclonal Antibody for WB, ELISA
Cat No. 13806-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Serine/threonine-protein kinase 22B, EC:2.7.11.1, DiGeorge syndrome protein G, DGS-G, DGSG

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13806-1-AP targets TSSK2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSSK2 fusion protein Ag4805 相同性解析による交差性が予測される生物種
Full Name testis-specific serine kinase 2
Calculated molecular weight 358 aa, 41 kDa
Observed molecular weight40 kDa
GenBank accession numberBC037781
Gene Symbol TSSK2
Gene ID (NCBI) 23617
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PF2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TSSK2 (Testis-Specific Serine/Threonine Kinase 2) is a member of the TSSK family, predominantly expressed in the testis and involved in spermiogenesis and male fertility. It is localized in various regions of sperm, including the centrioles of post-meiotic spermatids, the tail, acrosomal regions, and the midpiece. TSSK2 plays a crucial role in sperm maturation by phosphorylating substrates such as TSKS and SPAG16L. Studies have shown that TSSK2 knockout mice exhibit infertility due to impaired spermatid elongation and sperm maturation. Additionally, TSSK2 is considered a promising target for reversible male contraception, with several potent inhibitors identified through high-throughput screening.

Protocols

Product Specific Protocols
WB protocol for TSSK2 antibody 13806-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols