Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19852-1-AP targets TSSC1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13923 Product name: Recombinant human TSSC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-387 aa of BC002485 Sequence: MEDDAPVIYGLEFQARALTPQTAETDAIRFLVGTQSLKYDNQIHIIDFDDENNIINKNVLLHQAGEIWHISASPADRGVLTTCYNRTSDSKVLTCAAVWRMPKELESGSHESPDDSSSTAQTLELLCHLDNTAHGNMACVVWEPMGDGKKIISLADNHILLWDLQESSSQAVLASSASLEGKGQLKFTSGRWSPHHNCTQVATANDTTLRGWDTRSMSQIYCIENAHGQLVRDLDFNPNKQYYLASCGDDCKVKFWDTRNVTEPVKTLEEHSHWVWNVRYNHSHDQLVLTGSSDSRVILSNMVSISSEPFGHLVDDDDISDQEDHRSEEKSKEPLQDNVIATYEEHEDSVYAVDWSSADPWLFASLSYDGRLVINRVPRALKYHILL 相同性解析による交差性が予測される生物種 |
| Full Name | tumor suppressing subtransferable candidate 1 |
| Calculated molecular weight | 387 aa, 44 kDa |
| Observed molecular weight | ~50 kDa |
| GenBank accession number | BC002485 |
| Gene Symbol | TSSC1 |
| Gene ID (NCBI) | 7260 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q53HC9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSSC1 (Tumor Suppressing Subtransferable Candidate 1), now officially designated EIPR1, encodes a 387-amino-acid WD40 repeat protein that localizes to chromosome 2p25.3 in humans. Although originally identified within a putative tumor-suppressor region at 11p15.5, later genomic mapping reassigned the gene to chromosome 2. Functionally, TSSC1 is a component of the endosomal retrieval machinery, interacting with both the EARP and GARP tethering complexes to regulate protein transport from early endosomes to the trans-Golgi network and recycling endosomes. Gershlick et al. demonstrated that TSSC1 is required for efficient recruitment of GARP to the trans-Golgi network and functions as a regulator of both GARP and EARP in endosomal retrieval pathways (PMID: 27440922).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSSC1 antibody 19852-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |