Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HeLa using 16263-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 16263-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 16263-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human spleen using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human epididymis using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human epididymis using 16263-1-AP
Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of MCF-7 using 16263-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TSR2 antibody (16263-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

TSR2 Polyclonal antibody

TSR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16263-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WGG1, Pre-rRNA-processing protein TSR2 homolog, DT1P1A10

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, mouse kidney tissue
Positive IHC detected in	human liver tissue, human epididymis tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 2 publications below

Product Information

16263-1-AP targets TSR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9328 Product name: Recombinant human TSR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-191 aa of BC011825 Sequence: MAGAAEDARALFRAGVCAALEAWPALQIAVENGFGGVHSQEKAKWLGGAVEDYFMRNADLELDEVEDFLGELLTNEFDTVVEDGSLPQVSQQLQTMFHHFQRGDGAALREMASCITQRKCKVTATALKTARETDEDEDDVDSVEEMEVTATNDGAATDGVCPQPEPSDPDAQTIKEEDIVEDGWTIVRRKK 相同性解析による交差性が予測される生物種
Full Name	TSR2, 20S rRNA accumulation, homolog (S. cerevisiae)
Calculated molecular weight	191 aa, 21 kDa
Observed molecular weight	24-30 kDa
GenBank accession number	BC011825
Gene Symbol	TSR2
Gene ID (NCBI)	90121
RRID	AB_2211381
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q969E8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TSR2 antibody 16263-1-AP	Download protocol
IHC protocol for TSR2 antibody 16263-1-AP	Download protocol
IF protocol for TSR2 antibody 16263-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Acta Histochem Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers. Authors - Ekaterina S Snigirevskaya View Article
human	IHC	Cell Biol Int Ultrastructural traits of apoptosis Authors - Ekaterina S Snigirevskaya View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 16263-1-AP

WB analysis of HEK-293 using 16263-1-AP

WB analysis of mouse kidney using 16263-1-AP

IHC staining of human liver using 16263-1-AP

IHC staining of human liver using 16263-1-AP

IHC staining of human spleen using 16263-1-AP

IHC staining of human spleen using 16263-1-AP

IHC staining of human heart using 16263-1-AP

IHC staining of human heart using 16263-1-AP

IHC staining of human lung using 16263-1-AP

IHC staining of human lung using 16263-1-AP

IHC staining of human kidney using 16263-1-AP