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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSR2 Polyclonal antibody

TSR2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16263-1-AP

Synonyms

DT1P1A10, TSR2, WGG1

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 16263-1-AP

    WB analysis of HeLa using 16263-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16263-1-AP

    WB analysis of HEK-293 using 16263-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 16263-1-AP

    WB analysis of mouse kidney using 16263-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16263-1-AP (TSR2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16263-1-AP

    IHC staining of human liver using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16263-1-AP

    IHC staining of human liver using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human spleen using 16263-1-AP

    IHC staining of human spleen using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human spleen using 16263-1-AP

    IHC staining of human spleen using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung using 16263-1-AP

    IHC staining of human lung using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 16263-1-AP

    IHC staining of human lung using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human epididymis using 16263-1-AP

    IHC staining of human epididymis using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human epididymis using 16263-1-AP

    IHC staining of human epididymis using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human epididymis using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human placenta using 16263-1-AP

    IHC staining of human placenta using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 16263-1-AP

    IHC staining of human placenta using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 16263-1-AP

    IHC staining of human heart using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16263-1-AP

    IHC staining of human heart using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 16263-1-AP

    IHC staining of human kidney using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 16263-1-AP

    IHC staining of human kidney using 16263-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16263-1-AP (TSR2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 16263-1-AP

    IF Staining of MCF-7 using 16263-1-AP

    Immunofluorescent analysis of MCF-7 cells, using TSR2 antibody 16263-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, mouse kidney tissue
Positive IHC detected inhuman liver tissue, human epididymis tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

16263-1-AP targets TSR2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TSR2 fusion protein Ag9328 相同性解析による交差性が予測される生物種
Full Name TSR2, 20S rRNA accumulation, homolog (S. cerevisiae)
Calculated molecular weight 191 aa, 21 kDa
Observed molecular weight 24-30 kDa
GenBank accession numberBC011825
Gene symbol TSR2
Gene ID (NCBI) 90121
RRIDAB_2211381
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TSR2 antibody 16263-1-APDownload protocol
IHC protocol for TSR2 antibody 16263-1-APDownload protocol
IF protocol for TSR2 antibody 16263-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Acta Histochem

Ultrastructural analysis of human leukemia U-937 cells after apoptosis induction: Localization of proteasomes and perichromatin fibers.

Authors - Ekaterina S Snigirevskaya
View Article
humanIHC

Cell Biol Int

Ultrastructural traits of apoptosis

Authors - Ekaterina S Snigirevskaya
View Article